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Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat
Most eukaryotic species are colonized by a microbial community – the microbiota – that is acquired during early life stages and is critical to host development and health. Much research has focused on the microbiota biodiversity during the host life, however, empirical data on the basic ecological p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861309/ https://www.ncbi.nlm.nih.gov/pubmed/29593687 http://dx.doi.org/10.3389/fmicb.2018.00443 |
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author | Wein, Tanita Dagan, Tal Fraune, Sebastian Bosch, Thomas C. G. Reusch, Thorsten B. H. Hülter, Nils F. |
author_facet | Wein, Tanita Dagan, Tal Fraune, Sebastian Bosch, Thomas C. G. Reusch, Thorsten B. H. Hülter, Nils F. |
author_sort | Wein, Tanita |
collection | PubMed |
description | Most eukaryotic species are colonized by a microbial community – the microbiota – that is acquired during early life stages and is critical to host development and health. Much research has focused on the microbiota biodiversity during the host life, however, empirical data on the basic ecological principles that govern microbiota assembly is lacking. Here we quantify the contribution of colonizer order, arrival time and colonization history to microbiota assembly on a host. We established the freshwater polyp Hydra vulgaris and its dominant colonizer Curvibacter as a model system that enables the visualization and quantification of colonizer population size at the single cell resolution, in vivo, in real time. We estimate the carrying capacity of a single Hydra polyp as 2 × 10(5) Curvibacter cells, which is robust among individuals and time. Colonization experiments reveal a clear priority effect of first colonizers that depends on arrival time and colonization history. First arriving colonizers achieve a numerical advantage over secondary colonizers within a short time lag of 24 h. Furthermore, colonizers primed for the Hydra habitat achieve a numerical advantage in the absence of a time lag. These results follow the theoretical expectations for any bacterial habitat with a finite carrying capacity. Thus, Hydra colonization and succession processes are largely determined by the habitat occupancy over time and Curvibacter colonization history. Our experiments provide empirical data on the basic steps of host-associated microbiota establishment – the colonization stage. The presented approach supplies a framework for studying habitat characteristics and colonization dynamics within the host–microbe setting. |
format | Online Article Text |
id | pubmed-5861309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58613092018-03-28 Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat Wein, Tanita Dagan, Tal Fraune, Sebastian Bosch, Thomas C. G. Reusch, Thorsten B. H. Hülter, Nils F. Front Microbiol Microbiology Most eukaryotic species are colonized by a microbial community – the microbiota – that is acquired during early life stages and is critical to host development and health. Much research has focused on the microbiota biodiversity during the host life, however, empirical data on the basic ecological principles that govern microbiota assembly is lacking. Here we quantify the contribution of colonizer order, arrival time and colonization history to microbiota assembly on a host. We established the freshwater polyp Hydra vulgaris and its dominant colonizer Curvibacter as a model system that enables the visualization and quantification of colonizer population size at the single cell resolution, in vivo, in real time. We estimate the carrying capacity of a single Hydra polyp as 2 × 10(5) Curvibacter cells, which is robust among individuals and time. Colonization experiments reveal a clear priority effect of first colonizers that depends on arrival time and colonization history. First arriving colonizers achieve a numerical advantage over secondary colonizers within a short time lag of 24 h. Furthermore, colonizers primed for the Hydra habitat achieve a numerical advantage in the absence of a time lag. These results follow the theoretical expectations for any bacterial habitat with a finite carrying capacity. Thus, Hydra colonization and succession processes are largely determined by the habitat occupancy over time and Curvibacter colonization history. Our experiments provide empirical data on the basic steps of host-associated microbiota establishment – the colonization stage. The presented approach supplies a framework for studying habitat characteristics and colonization dynamics within the host–microbe setting. Frontiers Media S.A. 2018-03-14 /pmc/articles/PMC5861309/ /pubmed/29593687 http://dx.doi.org/10.3389/fmicb.2018.00443 Text en Copyright © 2018 Wein, Dagan, Fraune, Bosch, Reusch and Hülter. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wein, Tanita Dagan, Tal Fraune, Sebastian Bosch, Thomas C. G. Reusch, Thorsten B. H. Hülter, Nils F. Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat |
title | Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat |
title_full | Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat |
title_fullStr | Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat |
title_full_unstemmed | Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat |
title_short | Carrying Capacity and Colonization Dynamics of Curvibacter in the Hydra Host Habitat |
title_sort | carrying capacity and colonization dynamics of curvibacter in the hydra host habitat |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861309/ https://www.ncbi.nlm.nih.gov/pubmed/29593687 http://dx.doi.org/10.3389/fmicb.2018.00443 |
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