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Spermine modulates fungal morphogenesis and activates plasma membrane H(+)-ATPase during yeast to hyphae transition

Polyamines play a regulatory role in eukaryotic cell growth and morphogenesis. Despite many molecular advances, the underlying mechanism of action remains unclear. Here, we investigate a mechanism by which spermine affects the morphogenesis of a dimorphic fungal model of emerging relevance in plant...

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Detalles Bibliográficos
Autores principales: Cogo, Antônio Jesus Dorighetto, Dutra Ferreira, Keilla dos Reis, Okorokov, Lev A., Ramos, Alessandro C., Façanha, Arnoldo R., Okorokova-Façanha, Anna L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861359/
https://www.ncbi.nlm.nih.gov/pubmed/29361612
http://dx.doi.org/10.1242/bio.029660
Descripción
Sumario:Polyamines play a regulatory role in eukaryotic cell growth and morphogenesis. Despite many molecular advances, the underlying mechanism of action remains unclear. Here, we investigate a mechanism by which spermine affects the morphogenesis of a dimorphic fungal model of emerging relevance in plant interactions, Yarrowia lipolytica, through the recruitment of a phytohormone-like pathway involving activation of the plasma membrane P-type H(+)-ATPase. Morphological transition was followed microscopically, and the H(+)-ATPase activity was analyzed in isolated membrane vesicles. Proton flux and acidification were directly probed at living cell surfaces by a non-invasive selective ion electrode technique. Spermine and indol-3-acetic acid (IAA) induced the yeast-hypha transition, influencing the colony architecture. Spermine induced H(+)-ATPase activity and H(+) efflux in living cells correlating with yeast-hypha dynamics. Pharmacological inhibition of spermine and IAA pathways prevented the physio-morphological responses, and indicated that spermine could act upstream of the IAA pathway. This study provides the first compelling evidence on the fungal morphogenesis and colony development as modulated by a spermine-induced acid growth mechanism analogous to that previously postulated for the multicellular growth regulation of plants.