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ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes
The exponentially increasing volumes of DNA sequence data highlight the need for new DNA cloning methods to explore the new information. Here, we describe ‘ExoCET’ (Exonuclease Combined with RecET recombination) to directly clone any chosen region from bacterial and mammalian genomes with nucleotide...
Autores principales: | Wang, Hailong, Li, Zhen, Jia, Ruonan, Yin, Jia, Li, Aiying, Xia, Liqiu, Yin, Yulong, Müller, Rolf, Fu, Jun, Stewart, A Francis, Zhang, Youming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861427/ https://www.ncbi.nlm.nih.gov/pubmed/29240926 http://dx.doi.org/10.1093/nar/gkx1249 |
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