ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes

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The exponentially increasing volumes of DNA sequence data highlight the need for new DNA cloning methods to explore the new information. Here, we describe ‘ExoCET’ (Exonuclease Combined with RecET recombination) to directly clone any chosen region from bacterial and mammalian genomes with nucleotide...

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Detalles Bibliográficos
Autores principales: Wang, Hailong, Li, Zhen, Jia, Ruonan, Yin, Jia, Li, Aiying, Xia, Liqiu, Yin, Yulong, Müller, Rolf, Fu, Jun, Stewart, A Francis, Zhang, Youming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861427/
https://www.ncbi.nlm.nih.gov/pubmed/29240926
http://dx.doi.org/10.1093/nar/gkx1249
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