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Structural and functional adaptation of Haloferax volcanii TFEα/β

The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in...

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Autores principales: Blombach, Fabian, Ausiannikava, Darya, Figueiredo, Angelo Miguel, Soloviev, Zoja, Prentice, Tanya, Zhang, Mark, Zhou, Nanruoyi, Thalassinos, Konstantinos, Allers, Thorsten, Werner, Finn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861453/
https://www.ncbi.nlm.nih.gov/pubmed/29309690
http://dx.doi.org/10.1093/nar/gkx1302
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author Blombach, Fabian
Ausiannikava, Darya
Figueiredo, Angelo Miguel
Soloviev, Zoja
Prentice, Tanya
Zhang, Mark
Zhou, Nanruoyi
Thalassinos, Konstantinos
Allers, Thorsten
Werner, Finn
author_facet Blombach, Fabian
Ausiannikava, Darya
Figueiredo, Angelo Miguel
Soloviev, Zoja
Prentice, Tanya
Zhang, Mark
Zhou, Nanruoyi
Thalassinos, Konstantinos
Allers, Thorsten
Werner, Finn
author_sort Blombach, Fabian
collection PubMed
description The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit. Halophilic archaea such as Haloferax volcanii have highly divergent putative TFEβ homologues with unknown properties. Here, we demonstrate that Haloferax TFEβ lacks the prototypical iron-sulphur cluster yet still forms a stable complex with TFEα. A second metal cluster contained in the zinc ribbon domain in TFEα is highly degenerate but retains low binding affinity for zinc, which contributes to protein folding and stability. The deletion of the tfeB gene in H. volcanii results in the aberrant expression of approximately one third of all genes, consistent with its function as a basal transcription initiation factor. Interestingly, tfeB deletion particularly affects foreign genes including a prophage region. Our results reveal the loss of metal centres in Hvo transcription factors, and confirm the dual function of TFE as basal factor and regulator of transcription.
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spelling pubmed-58614532018-03-28 Structural and functional adaptation of Haloferax volcanii TFEα/β Blombach, Fabian Ausiannikava, Darya Figueiredo, Angelo Miguel Soloviev, Zoja Prentice, Tanya Zhang, Mark Zhou, Nanruoyi Thalassinos, Konstantinos Allers, Thorsten Werner, Finn Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit. Halophilic archaea such as Haloferax volcanii have highly divergent putative TFEβ homologues with unknown properties. Here, we demonstrate that Haloferax TFEβ lacks the prototypical iron-sulphur cluster yet still forms a stable complex with TFEα. A second metal cluster contained in the zinc ribbon domain in TFEα is highly degenerate but retains low binding affinity for zinc, which contributes to protein folding and stability. The deletion of the tfeB gene in H. volcanii results in the aberrant expression of approximately one third of all genes, consistent with its function as a basal transcription initiation factor. Interestingly, tfeB deletion particularly affects foreign genes including a prophage region. Our results reveal the loss of metal centres in Hvo transcription factors, and confirm the dual function of TFE as basal factor and regulator of transcription. Oxford University Press 2018-03-16 2018-01-04 /pmc/articles/PMC5861453/ /pubmed/29309690 http://dx.doi.org/10.1093/nar/gkx1302 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene regulation, Chromatin and Epigenetics
Blombach, Fabian
Ausiannikava, Darya
Figueiredo, Angelo Miguel
Soloviev, Zoja
Prentice, Tanya
Zhang, Mark
Zhou, Nanruoyi
Thalassinos, Konstantinos
Allers, Thorsten
Werner, Finn
Structural and functional adaptation of Haloferax volcanii TFEα/β
title Structural and functional adaptation of Haloferax volcanii TFEα/β
title_full Structural and functional adaptation of Haloferax volcanii TFEα/β
title_fullStr Structural and functional adaptation of Haloferax volcanii TFEα/β
title_full_unstemmed Structural and functional adaptation of Haloferax volcanii TFEα/β
title_short Structural and functional adaptation of Haloferax volcanii TFEα/β
title_sort structural and functional adaptation of haloferax volcanii tfeα/β
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861453/
https://www.ncbi.nlm.nih.gov/pubmed/29309690
http://dx.doi.org/10.1093/nar/gkx1302
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