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(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes

CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization using super-resolution microscopy. We developed (Po)STAC (Polycistronic SunT...

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Detalles Bibliográficos
Autores principales:	Neguembor, Maria V, Sebastian-Perez, Ruben, Aulicino, Francesco, Gomez-Garcia, Pablo A, Cosma, Maria P, Lakadamyali, Melike
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861460/ https://www.ncbi.nlm.nih.gov/pubmed/29294098 http://dx.doi.org/10.1093/nar/gkx1271

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