Isotope Substitution of Promiscuous Alcohol Dehydrogenase Reveals the Origin of Substrate Preference in the Transition State

The origin of substrate preference in promiscuous enzymes was investigated by enzyme isotope labelling of the alcohol dehydrogenase from Geobacillus stearothermophilus (BsADH). At physiological temperature, protein dynamic coupling to the reaction coordinate was insignificant. However, the extent of...

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Detalles Bibliográficos
Autores principales: Behiry, Enas M., Ruiz‐Pernia, J. Javier, Luk, Louis, Tuñón, Iñaki, Moliner, Vicent, Allemann, Rudolf K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861672/
https://www.ncbi.nlm.nih.gov/pubmed/29341402
http://dx.doi.org/10.1002/anie.201712826

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861672/
https://www.ncbi.nlm.nih.gov/pubmed/29341402
http://dx.doi.org/10.1002/anie.201712826

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