Cargando…

Isotope Substitution of Promiscuous Alcohol Dehydrogenase Reveals the Origin of Substrate Preference in the Transition State

The origin of substrate preference in promiscuous enzymes was investigated by enzyme isotope labelling of the alcohol dehydrogenase from Geobacillus stearothermophilus (BsADH). At physiological temperature, protein dynamic coupling to the reaction coordinate was insignificant. However, the extent of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Behiry, Enas M., Ruiz‐Pernia, J. Javier, Luk, Louis, Tuñón, Iñaki, Moliner, Vicent, Allemann, Rudolf K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2018
Materias:	Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861672/ https://www.ncbi.nlm.nih.gov/pubmed/29341402 http://dx.doi.org/10.1002/anie.201712826

Ejemplares similares

Minimization of dynamic effects in the evolution of dihydrofolate reductase
por: Ruiz-Pernía, J. Javier, et al.
Publicado: (2016)

Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase
por: Luk, Louis Y. P., et al.
Publicado: (2015)

Loss of Hyperconjugative Effects Drives Hydride Transfer during Dihydrofolate Reductase Catalysis
por: Angelastro, Antonio, et al.
Publicado: (2019)

Why Are Some Enzymes Dimers? Flexibility and Catalysis in Thermotoga maritima Dihydrofolate Reductase
por: Ruiz-Pernía, J. Javier, et al.
Publicado: (2019)

Heavy Enzymes and the Rational Redesign of Protein Catalysts
por: Scott, Alan F., et al.
Publicado: (2019)

Increased Dynamic Effects in a Catalytically Compromised Variant of Escherichia coli Dihydrofolate Reductase
por: Ruiz-Pernia, J. Javier, et al.
Publicado: (2013)

Electric Field Measurements Reveal the Pivotal Role of Cofactor–Substrate Interaction in Dihydrofolate Reductase Catalysis
por: Adesina, Aduragbemi S., et al.
Publicado: (2020)

Combined Theoretical and Experimental Study to Unravel the Differences in Promiscuous Amidase Activity of Two Nonhomologous Enzymes
por: Galmés, Miquel À., et al.
Publicado: (2021)

Computational design of an amidase by combining the best electrostatic features of two promiscuous hydrolases
por: Galmés, Miquel À., et al.
Publicado: (2022)

Is Promiscuous CALB a Good Scaffold for Designing New Epoxidases?
por: Bordes, Isabel, et al.
Publicado: (2015)

Engineering substrate promiscuity in halophilic alcohol dehydrogenase (HvADH2) by in silico design
por: Cassidy, Jennifer, et al.
Publicado: (2017)

Structural insights into the enzymatic activity and potential substrate promiscuity of human 3-phosphoglycerate dehydrogenase (PHGDH)
por: Unterlass, Judith E., et al.
Publicado: (2017)

Silent catalytic promiscuity in the high-fidelity terpene cyclase δ-cadinene synthase
por: Loizzi, Marianna, et al.
Publicado: (2019)

Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme
por: Fürst, Maximilian J. L. J., et al.
Publicado: (2018)

Promiscuity and preferences of metallothioneins: the cell rules
por: Foster, Andrew W, et al.
Publicado: (2011)

NRPS Substrate Promiscuity Diversifies the Xenematides
por: Crawford, Jason M., et al.
Publicado: (2011)

Substrate Promiscuity of N-Acetylhexosamine 1-Kinases
por: Li, Yanhong, et al.
Publicado: (2011)

MTH1 Substrate Recognition—An Example of Specific Promiscuity
por: Nissink, J. Willem M., et al.
Publicado: (2016)

Discovery of novel geranylgeranyl reductases and characterization of their substrate promiscuity
por: Meadows, Corey W., et al.
Publicado: (2018)

Enzyme promiscuity shapes adaptation to novel growth substrates
por: Guzmán, Gabriela I, et al.
Publicado: (2019)

Inhibition Mechanism of SARS‐CoV‐2 Main Protease with Ketone‐Based Inhibitors Unveiled by Multiscale Simulations: Insights for Improved Designs
por: Ramos‐Guzmán, Carlos A., et al.
Publicado: (2021)

A microscopic description of SARS-CoV-2 main protease inhibition with Michael acceptors. Strategies for improving inhibitor design
por: Ramos-Guzmán, Carlos A., et al.
Publicado: (2021)

Substrate Interactions and Promiscuity in a Viral DNA Packaging Motor
por: Aathavan, K., et al.
Publicado: (2009)

Insights into substrate promiscuity of human seryl-tRNA synthetase
por: Holman, Kaitlyn M., et al.
Publicado: (2017)

Biological Filtering and Substrate Promiscuity Prediction for Annotating Untargeted Metabolomics
por: Hassanpour, Neda, et al.
Publicado: (2020)

Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis
por: McDonald, Allwin D., et al.
Publicado: (2022)

Biocatalytic Cascades toward Iminosugar Scaffolds Reveal Promiscuous Activity of Shikimate Dehydrogenases
por: Swanson, Christopher R. B., et al.
Publicado: (2023)

Mechanistic Insight into the Catalytic Promiscuity of Amine Dehydrogenases: Asymmetric Synthesis of Secondary and Primary Amines
por: Tseliou, Vasilis, et al.
Publicado: (2019)

Exploiting Substrate Promiscuity of Ectoine Hydroxylase for Regio- and Stereoselective Modification of Homoectoine
por: Czech, Laura, et al.
Publicado: (2019)

Engineering substrate promiscuity in 2,4-dichlorophenol hydroxylase by in silico design
por: Wang, Ye, et al.
Publicado: (2018)

Structural basis of promiscuous substrate transport by Organic Cation Transporter 1
por: Zeng, Yi C., et al.
Publicado: (2023)

Structural polymorphism and substrate promiscuity of a ribosome-associated molecular chaperone
por: Huang, Chih-Ting, et al.
Publicado: (2021)

Unveiling the Mechanistic Singularities of Caspases: A Computational Analysis of the Reaction Mechanism in Human Caspase-1
por: Ramos-Guzmán, Carlos A., et al.
Publicado: (2023)

Transfer hydrogenations catalyzed by streptavidin-hosted secondary amine organocatalysts
por: Santi, Nicolò, et al.
Publicado: (2021)

Cryo‐kinetics Reveal Dynamic Effects on the Chemistry of Human Dihydrofolate Reductase
por: Adesina, Aduragbemi S., et al.
Publicado: (2021)

Theoretical insights on helix repacking as the origin of P-glycoprotein promiscuity
por: Bonito, Cátia A., et al.
Publicado: (2020)

Different Dynamical Effects in Mesophilic and Hyperthermophilic Dihydrofolate Reductases
por: Luk, Louis Y. P., et al.
Publicado: (2014)

Covariation and repeatability of male mating effort and mating preferences in a promiscuous fish
por: Godin, Jean-Guy J, et al.
Publicado: (2013)

Substrate Profiling of Anion Methyltransferases for Promiscuous Synthesis of S‐Adenosylmethionine Analogs from Haloalkanes
por: Schülke, Kai H., et al.
Publicado: (2022)

Nickel quercetinase, a “promiscuous” metalloenzyme: metal incorporation and metal ligand substitution studies
por: Nianios, Dimitrios, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_cql18682uchgssdl5s8vhbnrft