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A bright organic NIR-II nanofluorophore for three-dimensional imaging into biological tissues

Fluorescence imaging of biological systems in the second near-infrared (NIR-II, 1000–1700 nm) window has shown promise of high spatial resolution, low background, and deep tissue penetration owing to low autofluorescence and suppressed scattering of long wavelength photons. Here we develop a bright...

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Detalles Bibliográficos
Autores principales: Wan, Hao, Yue, Jingying, Zhu, Shoujun, Uno, Takaaki, Zhang, Xiaodong, Yang, Qinglai, Yu, Kuai, Hong, Guosong, Wang, Junying, Li, Lulin, Ma, Zhuoran, Gao, Hongpeng, Zhong, Yeteng, Su, Jessica, Antaris, Alexander L., Xia, Yan, Luo, Jian, Liang, Yongye, Dai, Hongjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862886/
https://www.ncbi.nlm.nih.gov/pubmed/29563581
http://dx.doi.org/10.1038/s41467-018-03505-4
Descripción
Sumario:Fluorescence imaging of biological systems in the second near-infrared (NIR-II, 1000–1700 nm) window has shown promise of high spatial resolution, low background, and deep tissue penetration owing to low autofluorescence and suppressed scattering of long wavelength photons. Here we develop a bright organic nanofluorophore (named p-FE) for high-performance biological imaging in the NIR-II window. The bright NIR-II >1100 nm fluorescence emission from p-FE affords non-invasive in vivo tracking of blood flow in mouse brain vessels. Excitingly, p-FE enables one-photon based, three-dimensional (3D) confocal imaging of vasculatures in fixed mouse brain tissue with a layer-by-layer imaging depth up to ~1.3 mm and sub-10 µm high spatial resolution. We also perform in vivo two-color fluorescence imaging in the NIR-II window by utilizing p-FE as a vasculature imaging agent emitting between 1100 and 1300 nm and single-walled carbon nanotubes (CNTs) emitting above 1500 nm to highlight tumors in mice.