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Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control

Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible for this event have not been fully identified in high eukaryotes. Here, we report a mammalian tRNA/rRN...

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Autores principales: Yang, Jin-Yu, Deng, Xiang-Yu, Li, Yi-Sheng, Ma, Xian-Cai, Feng, Jian-Xiong, Yu, Bing, Chen, Yang, Luo, Yi-Ling, Wang, Xi, Chen, Mei-Ling, Fang, Zhi-Xin, Zheng, Fu-Xiang, Li, Yi-Ping, Zhong, Qian, Kang, Tie-Bang, Song, Li-Bing, Xu, Rui-Hua, Zeng, Mu-Sheng, Chen, Wei, Zhang, Hui, Xie, Wei, Gao, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862951/
https://www.ncbi.nlm.nih.gov/pubmed/29563550
http://dx.doi.org/10.1038/s41467-018-03544-x
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author Yang, Jin-Yu
Deng, Xiang-Yu
Li, Yi-Sheng
Ma, Xian-Cai
Feng, Jian-Xiong
Yu, Bing
Chen, Yang
Luo, Yi-Ling
Wang, Xi
Chen, Mei-Ling
Fang, Zhi-Xin
Zheng, Fu-Xiang
Li, Yi-Ping
Zhong, Qian
Kang, Tie-Bang
Song, Li-Bing
Xu, Rui-Hua
Zeng, Mu-Sheng
Chen, Wei
Zhang, Hui
Xie, Wei
Gao, Song
author_facet Yang, Jin-Yu
Deng, Xiang-Yu
Li, Yi-Sheng
Ma, Xian-Cai
Feng, Jian-Xiong
Yu, Bing
Chen, Yang
Luo, Yi-Ling
Wang, Xi
Chen, Mei-Ling
Fang, Zhi-Xin
Zheng, Fu-Xiang
Li, Yi-Ping
Zhong, Qian
Kang, Tie-Bang
Song, Li-Bing
Xu, Rui-Hua
Zeng, Mu-Sheng
Chen, Wei
Zhang, Hui
Xie, Wei
Gao, Song
author_sort Yang, Jin-Yu
collection PubMed
description Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible for this event have not been fully identified in high eukaryotes. Here, we report a mammalian tRNA/rRNA-targeting endoribonuclease: SLFN13, a member of the Schlafen family. Structural study reveals a unique pseudo-dimeric U-pillow-shaped architecture of the SLFN13 N′-domain that may clamp base-paired RNAs. SLFN13 is able to digest tRNAs and rRNAs in vitro, and the endonucleolytic cleavage dissevers 11 nucleotides from the 3′-terminus of tRNA at the acceptor stem. The cytoplasmically localised SLFN13 inhibits protein synthesis in 293T cells. Moreover, SLFN13 restricts HIV replication in a nucleolytic activity-dependent manner. According to these observations, we term SLFN13 RNase S13. Our study provides insights into the modulation of translational machinery in high eukaryotes, and sheds light on the functional mechanisms of the Schlafen family.
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spelling pubmed-58629512018-03-23 Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control Yang, Jin-Yu Deng, Xiang-Yu Li, Yi-Sheng Ma, Xian-Cai Feng, Jian-Xiong Yu, Bing Chen, Yang Luo, Yi-Ling Wang, Xi Chen, Mei-Ling Fang, Zhi-Xin Zheng, Fu-Xiang Li, Yi-Ping Zhong, Qian Kang, Tie-Bang Song, Li-Bing Xu, Rui-Hua Zeng, Mu-Sheng Chen, Wei Zhang, Hui Xie, Wei Gao, Song Nat Commun Article Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible for this event have not been fully identified in high eukaryotes. Here, we report a mammalian tRNA/rRNA-targeting endoribonuclease: SLFN13, a member of the Schlafen family. Structural study reveals a unique pseudo-dimeric U-pillow-shaped architecture of the SLFN13 N′-domain that may clamp base-paired RNAs. SLFN13 is able to digest tRNAs and rRNAs in vitro, and the endonucleolytic cleavage dissevers 11 nucleotides from the 3′-terminus of tRNA at the acceptor stem. The cytoplasmically localised SLFN13 inhibits protein synthesis in 293T cells. Moreover, SLFN13 restricts HIV replication in a nucleolytic activity-dependent manner. According to these observations, we term SLFN13 RNase S13. Our study provides insights into the modulation of translational machinery in high eukaryotes, and sheds light on the functional mechanisms of the Schlafen family. Nature Publishing Group UK 2018-03-21 /pmc/articles/PMC5862951/ /pubmed/29563550 http://dx.doi.org/10.1038/s41467-018-03544-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Jin-Yu
Deng, Xiang-Yu
Li, Yi-Sheng
Ma, Xian-Cai
Feng, Jian-Xiong
Yu, Bing
Chen, Yang
Luo, Yi-Ling
Wang, Xi
Chen, Mei-Ling
Fang, Zhi-Xin
Zheng, Fu-Xiang
Li, Yi-Ping
Zhong, Qian
Kang, Tie-Bang
Song, Li-Bing
Xu, Rui-Hua
Zeng, Mu-Sheng
Chen, Wei
Zhang, Hui
Xie, Wei
Gao, Song
Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control
title Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control
title_full Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control
title_fullStr Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control
title_full_unstemmed Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control
title_short Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase engaged in translational control
title_sort structure of schlafen13 reveals a new class of trna/rrna- targeting rnase engaged in translational control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862951/
https://www.ncbi.nlm.nih.gov/pubmed/29563550
http://dx.doi.org/10.1038/s41467-018-03544-x
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