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The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice

There are more than 2300 genes that are predominantly expressed in mouse testes. The role of hundreds of these genes has been studied in mouse spermatogenesis but still there are many genes whose function is unknown. Gene knockout (KO) strategy in mice is widely used for in vivo study of gene functi...

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Autores principales: Khan, Manan, Jabeen, Nazish, Khan, Teka, Hussain, Hafiz Muhammad Jafar, Ali, Asim, Khan, Ranjha, Jiang, Long, Li, Tao, Tao, Qizhao, Zhang, Xingxia, Yin, Hao, Yu, Changping, Jiang, Xiaohua, Shi, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862965/
https://www.ncbi.nlm.nih.gov/pubmed/29563520
http://dx.doi.org/10.1038/s41598-018-23176-x
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author Khan, Manan
Jabeen, Nazish
Khan, Teka
Hussain, Hafiz Muhammad Jafar
Ali, Asim
Khan, Ranjha
Jiang, Long
Li, Tao
Tao, Qizhao
Zhang, Xingxia
Yin, Hao
Yu, Changping
Jiang, Xiaohua
Shi, Qinghua
author_facet Khan, Manan
Jabeen, Nazish
Khan, Teka
Hussain, Hafiz Muhammad Jafar
Ali, Asim
Khan, Ranjha
Jiang, Long
Li, Tao
Tao, Qizhao
Zhang, Xingxia
Yin, Hao
Yu, Changping
Jiang, Xiaohua
Shi, Qinghua
author_sort Khan, Manan
collection PubMed
description There are more than 2300 genes that are predominantly expressed in mouse testes. The role of hundreds of these genes has been studied in mouse spermatogenesis but still there are many genes whose function is unknown. Gene knockout (KO) strategy in mice is widely used for in vivo study of gene function. The present study was designed to explore the function of the four genes: Tex37, Ccdc73, Prss55 and Nxt2, which were evolutionarily conserved in eutherians. We found that these genes had a testis-enriched expression pattern in mice except Nxt2. We knocked out these genes by CRISPR/Cas9 individually and found that all the KO mice had normal fertility with no detectable difference in testis/body weight ratios, epididymal sperm counts, as well as testicular and epididymal histology from wild type mice. Although these genes are evolutionarily conserved in eutherians including human and mouse, they are not individually essential for spermatogenesis, testis development and male fertility in mice in laboratory conditions. Our report of these fertile KO data could avoid the repetition and duplication of efforts which will help in prioritizing efforts to focus on genes that are indispensable for male reproduction.
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spelling pubmed-58629652018-03-27 The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice Khan, Manan Jabeen, Nazish Khan, Teka Hussain, Hafiz Muhammad Jafar Ali, Asim Khan, Ranjha Jiang, Long Li, Tao Tao, Qizhao Zhang, Xingxia Yin, Hao Yu, Changping Jiang, Xiaohua Shi, Qinghua Sci Rep Article There are more than 2300 genes that are predominantly expressed in mouse testes. The role of hundreds of these genes has been studied in mouse spermatogenesis but still there are many genes whose function is unknown. Gene knockout (KO) strategy in mice is widely used for in vivo study of gene function. The present study was designed to explore the function of the four genes: Tex37, Ccdc73, Prss55 and Nxt2, which were evolutionarily conserved in eutherians. We found that these genes had a testis-enriched expression pattern in mice except Nxt2. We knocked out these genes by CRISPR/Cas9 individually and found that all the KO mice had normal fertility with no detectable difference in testis/body weight ratios, epididymal sperm counts, as well as testicular and epididymal histology from wild type mice. Although these genes are evolutionarily conserved in eutherians including human and mouse, they are not individually essential for spermatogenesis, testis development and male fertility in mice in laboratory conditions. Our report of these fertile KO data could avoid the repetition and duplication of efforts which will help in prioritizing efforts to focus on genes that are indispensable for male reproduction. Nature Publishing Group UK 2018-03-21 /pmc/articles/PMC5862965/ /pubmed/29563520 http://dx.doi.org/10.1038/s41598-018-23176-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Khan, Manan
Jabeen, Nazish
Khan, Teka
Hussain, Hafiz Muhammad Jafar
Ali, Asim
Khan, Ranjha
Jiang, Long
Li, Tao
Tao, Qizhao
Zhang, Xingxia
Yin, Hao
Yu, Changping
Jiang, Xiaohua
Shi, Qinghua
The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice
title The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice
title_full The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice
title_fullStr The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice
title_full_unstemmed The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice
title_short The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice
title_sort evolutionarily conserved genes: tex37, ccdc73, prss55 and nxt2 are dispensable for fertility in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862965/
https://www.ncbi.nlm.nih.gov/pubmed/29563520
http://dx.doi.org/10.1038/s41598-018-23176-x
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