MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells

Sphingosine 1-phosphate (S1P) is an intercellular signaling molecule present in blood. Erythrocytes have a central role in maintaining the S1P concentration in the blood stream. We previously demonstrated that S1P is exported from erythrocytes by a glyburide-sensitive S1P transporter. However, the g...

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Autores principales: Kobayashi, Naoki, Kawasaki-Nishi, Shoko, Otsuka, Masato, Hisano, Yu, Yamaguchi, Akihito, Nishi, Tsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862976/
https://www.ncbi.nlm.nih.gov/pubmed/29563527
http://dx.doi.org/10.1038/s41598-018-23300-x
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author Kobayashi, Naoki
Kawasaki-Nishi, Shoko
Otsuka, Masato
Hisano, Yu
Yamaguchi, Akihito
Nishi, Tsuyoshi
author_facet Kobayashi, Naoki
Kawasaki-Nishi, Shoko
Otsuka, Masato
Hisano, Yu
Yamaguchi, Akihito
Nishi, Tsuyoshi
author_sort Kobayashi, Naoki
collection PubMed
description Sphingosine 1-phosphate (S1P) is an intercellular signaling molecule present in blood. Erythrocytes have a central role in maintaining the S1P concentration in the blood stream. We previously demonstrated that S1P is exported from erythrocytes by a glyburide-sensitive S1P transporter. However, the gene encoding the S1P transporter in erythrocytes is unknown. In this study, we found that the mouse erythroid cell line, MEDEP-E14, has S1P export activity and exhibits properties that are consistent with those of erythrocytes. Using microarray analysis of MEDEP-E14 cells and its parental cell line, E14TG2a, we identified several candidate genes for S1P export activity. Of those genes, only one gene, Mfsd2b, showed S1P transport activity. The properties of S1P release by MFSD2B were similar to those in erythrocytes. Moreover, knockout of MFSD2B in MEDEP-E14 cells decreased S1P export from the cells. These results strongly suggest that MFSD2B is a novel S1P transporter in erythroid cells.
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spelling pubmed-58629762018-03-27 MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells Kobayashi, Naoki Kawasaki-Nishi, Shoko Otsuka, Masato Hisano, Yu Yamaguchi, Akihito Nishi, Tsuyoshi Sci Rep Article Sphingosine 1-phosphate (S1P) is an intercellular signaling molecule present in blood. Erythrocytes have a central role in maintaining the S1P concentration in the blood stream. We previously demonstrated that S1P is exported from erythrocytes by a glyburide-sensitive S1P transporter. However, the gene encoding the S1P transporter in erythrocytes is unknown. In this study, we found that the mouse erythroid cell line, MEDEP-E14, has S1P export activity and exhibits properties that are consistent with those of erythrocytes. Using microarray analysis of MEDEP-E14 cells and its parental cell line, E14TG2a, we identified several candidate genes for S1P export activity. Of those genes, only one gene, Mfsd2b, showed S1P transport activity. The properties of S1P release by MFSD2B were similar to those in erythrocytes. Moreover, knockout of MFSD2B in MEDEP-E14 cells decreased S1P export from the cells. These results strongly suggest that MFSD2B is a novel S1P transporter in erythroid cells. Nature Publishing Group UK 2018-03-21 /pmc/articles/PMC5862976/ /pubmed/29563527 http://dx.doi.org/10.1038/s41598-018-23300-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kobayashi, Naoki
Kawasaki-Nishi, Shoko
Otsuka, Masato
Hisano, Yu
Yamaguchi, Akihito
Nishi, Tsuyoshi
MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells
title MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells
title_full MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells
title_fullStr MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells
title_full_unstemmed MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells
title_short MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells
title_sort mfsd2b is a sphingosine 1-phosphate transporter in erythroid cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862976/
https://www.ncbi.nlm.nih.gov/pubmed/29563527
http://dx.doi.org/10.1038/s41598-018-23300-x
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