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Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions
The transcriptional response of Staphylococcus aureus strain Newman to sunlight exposure was investigated under both oxic and anoxic conditions using RNA sequencing to gain insight into potential mechanisms of inactivation. S. aureus is a pathogenic bacterium detected at recreational beaches which c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863498/ https://www.ncbi.nlm.nih.gov/pubmed/29599752 http://dx.doi.org/10.3389/fmicb.2018.00249 |
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author | McClary, Jill S. Boehm, Alexandria B. |
author_facet | McClary, Jill S. Boehm, Alexandria B. |
author_sort | McClary, Jill S. |
collection | PubMed |
description | The transcriptional response of Staphylococcus aureus strain Newman to sunlight exposure was investigated under both oxic and anoxic conditions using RNA sequencing to gain insight into potential mechanisms of inactivation. S. aureus is a pathogenic bacterium detected at recreational beaches which can cause gastrointestinal illness and skin infections, and is of increasing public health concern. To investigate the S. aureus photostress response in oligotrophic seawater, S. aureus cultures were suspended in seawater and exposed to full spectrum simulated sunlight. Experiments were performed under oxic or anoxic conditions to gain insight into the effects of oxygen-mediated and non-oxygen-mediated inactivation mechanisms. Transcript abundance was measured after 6 h of sunlight exposure using RNA sequencing and was compared to transcript abundance in paired dark control experiments. Culturable S. aureus decayed following biphasic inactivation kinetics with initial decay rate constants of 0.1 and 0.03 m(2) kJ(−1) in oxic and anoxic conditions, respectively. RNA sequencing revealed that 71 genes had different transcript abundance in the oxic sunlit experiments compared to dark controls, and 18 genes had different transcript abundance in the anoxic sunlit experiments compared to dark controls. The majority of genes showed reduced transcript abundance in the sunlit experiments under both conditions. Three genes (ebpS, NWMN_0867, and NWMN_1608) were found to have the same transcriptional response to sunlight between both oxic and anoxic conditions. In the oxic condition, transcripts associated with porphyrin metabolism, nitrate metabolism, and membrane transport functions were increased in abundance during sunlight exposure. Results suggest that S. aureus responds differently to oxygen-dependent and oxygen-independent photostress, and that endogenous photosensitizers play an important role during oxygen-dependent indirect photoinactivation. |
format | Online Article Text |
id | pubmed-5863498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58634982018-03-29 Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions McClary, Jill S. Boehm, Alexandria B. Front Microbiol Microbiology The transcriptional response of Staphylococcus aureus strain Newman to sunlight exposure was investigated under both oxic and anoxic conditions using RNA sequencing to gain insight into potential mechanisms of inactivation. S. aureus is a pathogenic bacterium detected at recreational beaches which can cause gastrointestinal illness and skin infections, and is of increasing public health concern. To investigate the S. aureus photostress response in oligotrophic seawater, S. aureus cultures were suspended in seawater and exposed to full spectrum simulated sunlight. Experiments were performed under oxic or anoxic conditions to gain insight into the effects of oxygen-mediated and non-oxygen-mediated inactivation mechanisms. Transcript abundance was measured after 6 h of sunlight exposure using RNA sequencing and was compared to transcript abundance in paired dark control experiments. Culturable S. aureus decayed following biphasic inactivation kinetics with initial decay rate constants of 0.1 and 0.03 m(2) kJ(−1) in oxic and anoxic conditions, respectively. RNA sequencing revealed that 71 genes had different transcript abundance in the oxic sunlit experiments compared to dark controls, and 18 genes had different transcript abundance in the anoxic sunlit experiments compared to dark controls. The majority of genes showed reduced transcript abundance in the sunlit experiments under both conditions. Three genes (ebpS, NWMN_0867, and NWMN_1608) were found to have the same transcriptional response to sunlight between both oxic and anoxic conditions. In the oxic condition, transcripts associated with porphyrin metabolism, nitrate metabolism, and membrane transport functions were increased in abundance during sunlight exposure. Results suggest that S. aureus responds differently to oxygen-dependent and oxygen-independent photostress, and that endogenous photosensitizers play an important role during oxygen-dependent indirect photoinactivation. Frontiers Media S.A. 2018-02-23 /pmc/articles/PMC5863498/ /pubmed/29599752 http://dx.doi.org/10.3389/fmicb.2018.00249 Text en Copyright © 2018 McClary and Boehm. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology McClary, Jill S. Boehm, Alexandria B. Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions |
title | Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions |
title_full | Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions |
title_fullStr | Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions |
title_full_unstemmed | Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions |
title_short | Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions |
title_sort | transcriptional response of staphylococcus aureus to sunlight in oxic and anoxic conditions |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863498/ https://www.ncbi.nlm.nih.gov/pubmed/29599752 http://dx.doi.org/10.3389/fmicb.2018.00249 |
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