Cargando…
The effect of tides on nearshore environmental DNA
We can recover genetic information from organisms of all kinds using environmental sampling. In recent years, sequencing this environmental DNA (eDNA) has become a tractable means of surveying many species using water, air, or soil samples. The technique is beginning to become a core tool for ecolog...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863721/ https://www.ncbi.nlm.nih.gov/pubmed/29576982 http://dx.doi.org/10.7717/peerj.4521 |
_version_ | 1783308431162081280 |
---|---|
author | Kelly, Ryan P. Gallego, Ramón Jacobs-Palmer, Emily |
author_facet | Kelly, Ryan P. Gallego, Ramón Jacobs-Palmer, Emily |
author_sort | Kelly, Ryan P. |
collection | PubMed |
description | We can recover genetic information from organisms of all kinds using environmental sampling. In recent years, sequencing this environmental DNA (eDNA) has become a tractable means of surveying many species using water, air, or soil samples. The technique is beginning to become a core tool for ecologists, environmental scientists, and biologists of many kinds, but the temporal resolution of eDNA sampling is often unclear, limiting the ecological interpretations of the resulting datasets. Here, in a temporally and spatially replicated field study using ca. 313 bp of eukaryotic COI mtDNA as a marker, we find that nearshore organismal communities are largely consistent across tides. Our findings suggest that nearshore eDNA from both benthic and planktonic taxa tends to be endogenous to the site and water mass sampled, rather than changing with each tidal cycle. However, where physiochemical water mass characteristics change, we find that the relative contributions of a broad range of organisms to eDNA communities shift in concert. |
format | Online Article Text |
id | pubmed-5863721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58637212018-03-24 The effect of tides on nearshore environmental DNA Kelly, Ryan P. Gallego, Ramón Jacobs-Palmer, Emily PeerJ Biodiversity We can recover genetic information from organisms of all kinds using environmental sampling. In recent years, sequencing this environmental DNA (eDNA) has become a tractable means of surveying many species using water, air, or soil samples. The technique is beginning to become a core tool for ecologists, environmental scientists, and biologists of many kinds, but the temporal resolution of eDNA sampling is often unclear, limiting the ecological interpretations of the resulting datasets. Here, in a temporally and spatially replicated field study using ca. 313 bp of eukaryotic COI mtDNA as a marker, we find that nearshore organismal communities are largely consistent across tides. Our findings suggest that nearshore eDNA from both benthic and planktonic taxa tends to be endogenous to the site and water mass sampled, rather than changing with each tidal cycle. However, where physiochemical water mass characteristics change, we find that the relative contributions of a broad range of organisms to eDNA communities shift in concert. PeerJ Inc. 2018-03-19 /pmc/articles/PMC5863721/ /pubmed/29576982 http://dx.doi.org/10.7717/peerj.4521 Text en ©2018 Kelly et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biodiversity Kelly, Ryan P. Gallego, Ramón Jacobs-Palmer, Emily The effect of tides on nearshore environmental DNA |
title | The effect of tides on nearshore environmental DNA |
title_full | The effect of tides on nearshore environmental DNA |
title_fullStr | The effect of tides on nearshore environmental DNA |
title_full_unstemmed | The effect of tides on nearshore environmental DNA |
title_short | The effect of tides on nearshore environmental DNA |
title_sort | effect of tides on nearshore environmental dna |
topic | Biodiversity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863721/ https://www.ncbi.nlm.nih.gov/pubmed/29576982 http://dx.doi.org/10.7717/peerj.4521 |
work_keys_str_mv | AT kellyryanp theeffectoftidesonnearshoreenvironmentaldna AT gallegoramon theeffectoftidesonnearshoreenvironmentaldna AT jacobspalmeremily theeffectoftidesonnearshoreenvironmentaldna AT kellyryanp effectoftidesonnearshoreenvironmentaldna AT gallegoramon effectoftidesonnearshoreenvironmentaldna AT jacobspalmeremily effectoftidesonnearshoreenvironmentaldna |