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Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae
A chemical and microbiological characterization on Hermetia illucens larvae was carried out as well as an inactivation study of natural contaminating microorganisms and inoculated E. coli O157:H7 in black soldier larvae by using High Hydrostatic Pressure (250 to 400 MPa, for 1.5 to 15 min). Hermetia...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864016/ https://www.ncbi.nlm.nih.gov/pubmed/29566029 http://dx.doi.org/10.1371/journal.pone.0194477 |
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author | Kashiri, Mahboobeh Marin, Cuauhtemoc Garzón, Raquel Rosell, Cristina M. Rodrigo, Dolores Martínez, Antonio |
author_facet | Kashiri, Mahboobeh Marin, Cuauhtemoc Garzón, Raquel Rosell, Cristina M. Rodrigo, Dolores Martínez, Antonio |
author_sort | Kashiri, Mahboobeh |
collection | PubMed |
description | A chemical and microbiological characterization on Hermetia illucens larvae was carried out as well as an inactivation study of natural contaminating microorganisms and inoculated E. coli O157:H7 in black soldier larvae by using High Hydrostatic Pressure (250 to 400 MPa, for 1.5 to 15 min). Hermetia illucens was mainly composed of proteins (46.49%, d.m.) followed by fat (37.88%, d.m.). Larvae had a high contamination load of Total Aerobic Mesophilic bacteria (AMB) (1.58x10(7) cfu/g) and Enterobacteriaceae (1.15x10(6)cfu/g). The presence of pathogenic microorganism varied: no Listeria spp. were found, but Salmonella (1.15x10(6) cfu/g) and E. coli (7.08x10(5) cfu/g) were detected in the larvae extract. High Hydrostatic Pressure (HHP) was effective against natural contaminating yeasts and molds producing more than 5 log cycle reductions at 400 MPa for any of the times considered (2.5 to 7 min), but a low reduction of total microbial load was achieved. The inactivation level of larvae inoculated with E. coli O157:H7 varied. At 400 MPa for 7 min more than 5 log cycle reductions were achieved. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells, according to the Akaike index, was the Biphasic model. |
format | Online Article Text |
id | pubmed-5864016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58640162018-03-28 Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae Kashiri, Mahboobeh Marin, Cuauhtemoc Garzón, Raquel Rosell, Cristina M. Rodrigo, Dolores Martínez, Antonio PLoS One Research Article A chemical and microbiological characterization on Hermetia illucens larvae was carried out as well as an inactivation study of natural contaminating microorganisms and inoculated E. coli O157:H7 in black soldier larvae by using High Hydrostatic Pressure (250 to 400 MPa, for 1.5 to 15 min). Hermetia illucens was mainly composed of proteins (46.49%, d.m.) followed by fat (37.88%, d.m.). Larvae had a high contamination load of Total Aerobic Mesophilic bacteria (AMB) (1.58x10(7) cfu/g) and Enterobacteriaceae (1.15x10(6)cfu/g). The presence of pathogenic microorganism varied: no Listeria spp. were found, but Salmonella (1.15x10(6) cfu/g) and E. coli (7.08x10(5) cfu/g) were detected in the larvae extract. High Hydrostatic Pressure (HHP) was effective against natural contaminating yeasts and molds producing more than 5 log cycle reductions at 400 MPa for any of the times considered (2.5 to 7 min), but a low reduction of total microbial load was achieved. The inactivation level of larvae inoculated with E. coli O157:H7 varied. At 400 MPa for 7 min more than 5 log cycle reductions were achieved. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells, according to the Akaike index, was the Biphasic model. Public Library of Science 2018-03-22 /pmc/articles/PMC5864016/ /pubmed/29566029 http://dx.doi.org/10.1371/journal.pone.0194477 Text en © 2018 Kashiri et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kashiri, Mahboobeh Marin, Cuauhtemoc Garzón, Raquel Rosell, Cristina M. Rodrigo, Dolores Martínez, Antonio Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae |
title | Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae |
title_full | Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae |
title_fullStr | Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae |
title_full_unstemmed | Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae |
title_short | Use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated E. coli O157:H7 on Hermetia illucens larvae |
title_sort | use of high hydrostatic pressure to inactivate natural contaminating microorganisms and inoculated e. coli o157:h7 on hermetia illucens larvae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864016/ https://www.ncbi.nlm.nih.gov/pubmed/29566029 http://dx.doi.org/10.1371/journal.pone.0194477 |
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