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Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter
Myeloblastosis (MYB) transcription factors play central roles in plant developmental processes and in responses to nutrient deficiency. In this study, OsMYB5P, an R2R3-MYB transcription factor, was isolated and identified from rice (Oryza sativa L. ‘Dongjin’) under inorganic phosphate (Pi)-deficient...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864048/ https://www.ncbi.nlm.nih.gov/pubmed/29566032 http://dx.doi.org/10.1371/journal.pone.0194628 |
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author | Yang, Won Tae Baek, Dongwon Yun, Dae-Jin Lee, Kwang Sik Hong, So Yeon Bae, Ki Deuk Chung, Young Soo Kwon, Yong Sham Kim, Du Hyun Jung, Ki Hong Kim, Doh Hoon |
author_facet | Yang, Won Tae Baek, Dongwon Yun, Dae-Jin Lee, Kwang Sik Hong, So Yeon Bae, Ki Deuk Chung, Young Soo Kwon, Yong Sham Kim, Du Hyun Jung, Ki Hong Kim, Doh Hoon |
author_sort | Yang, Won Tae |
collection | PubMed |
description | Myeloblastosis (MYB) transcription factors play central roles in plant developmental processes and in responses to nutrient deficiency. In this study, OsMYB5P, an R2R3-MYB transcription factor, was isolated and identified from rice (Oryza sativa L. ‘Dongjin’) under inorganic phosphate (Pi)-deficient conditions. OsMYB5P protein is localized to the nucleus and functions as a transcription activator in plant development. Overexpression of OsMYB5P in rice and Arabidopsis (Arabidopsis thaliana Col-0) increases tolerance to phosphate starvation, whereas OsMYB5P knock-out through RNA interference increases sensitivity to Pi depletion in rice. Furthermore, shoots and roots of transgenic rice plants overexpressing OsMYB5P were longer than those of wild plants under both normal and Pi-deficient conditions. These results indicate that OsMYB5P is associated with the regulation of shoot development and root- system architecture. Overexpression of OsMYB5P led to increased Pi accumulation in shoots and roots. Interestingly, OsMYB5P directly bound to MBS (MYB binding site) motifs on the OsPT5 promoter and induced transcription of OsPT5 in rice. In addition, overexpression of OsMYB5P in Arabidopsis triggered increased expression of AtPht1;3, an Arabidopsis Pi transporter, in shoots and roots under normal and Pi-deficient conditions. Together, these results demonstrate that overexpression of OsMYB5P increases tolerance to Pi deficiency in plants by modulating Pi transporters at the transcriptional level in monocots and dicots. |
format | Online Article Text |
id | pubmed-5864048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58640482018-03-28 Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter Yang, Won Tae Baek, Dongwon Yun, Dae-Jin Lee, Kwang Sik Hong, So Yeon Bae, Ki Deuk Chung, Young Soo Kwon, Yong Sham Kim, Du Hyun Jung, Ki Hong Kim, Doh Hoon PLoS One Research Article Myeloblastosis (MYB) transcription factors play central roles in plant developmental processes and in responses to nutrient deficiency. In this study, OsMYB5P, an R2R3-MYB transcription factor, was isolated and identified from rice (Oryza sativa L. ‘Dongjin’) under inorganic phosphate (Pi)-deficient conditions. OsMYB5P protein is localized to the nucleus and functions as a transcription activator in plant development. Overexpression of OsMYB5P in rice and Arabidopsis (Arabidopsis thaliana Col-0) increases tolerance to phosphate starvation, whereas OsMYB5P knock-out through RNA interference increases sensitivity to Pi depletion in rice. Furthermore, shoots and roots of transgenic rice plants overexpressing OsMYB5P were longer than those of wild plants under both normal and Pi-deficient conditions. These results indicate that OsMYB5P is associated with the regulation of shoot development and root- system architecture. Overexpression of OsMYB5P led to increased Pi accumulation in shoots and roots. Interestingly, OsMYB5P directly bound to MBS (MYB binding site) motifs on the OsPT5 promoter and induced transcription of OsPT5 in rice. In addition, overexpression of OsMYB5P in Arabidopsis triggered increased expression of AtPht1;3, an Arabidopsis Pi transporter, in shoots and roots under normal and Pi-deficient conditions. Together, these results demonstrate that overexpression of OsMYB5P increases tolerance to Pi deficiency in plants by modulating Pi transporters at the transcriptional level in monocots and dicots. Public Library of Science 2018-03-22 /pmc/articles/PMC5864048/ /pubmed/29566032 http://dx.doi.org/10.1371/journal.pone.0194628 Text en © 2018 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Yang, Won Tae Baek, Dongwon Yun, Dae-Jin Lee, Kwang Sik Hong, So Yeon Bae, Ki Deuk Chung, Young Soo Kwon, Yong Sham Kim, Du Hyun Jung, Ki Hong Kim, Doh Hoon Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter |
title | Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter |
title_full | Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter |
title_fullStr | Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter |
title_full_unstemmed | Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter |
title_short | Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter |
title_sort | rice osmyb5p improves plant phosphate acquisition by regulation of phosphate transporter |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864048/ https://www.ncbi.nlm.nih.gov/pubmed/29566032 http://dx.doi.org/10.1371/journal.pone.0194628 |
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