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Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise
Interactions between co-existing microorganisms deeply affect the physiology of the involved organisms and, ultimately, the function of the ecosystem as a whole. Copiotrophic Alteromonas are marine gammaproteobacteria that thrive during the late stages of phytoplankton blooms in the marine environme...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864184/ https://www.ncbi.nlm.nih.gov/pubmed/29335641 http://dx.doi.org/10.1038/s41396-017-0034-4 |
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author | Hou, Shengwei López-Pérez, Mario Pfreundt, Ulrike Belkin, Natalia Stüber, Kurt Huettel, Bruno Reinhardt, Richard Berman-Frank, Ilana Rodriguez-Valera, Francisco Hess, Wolfgang R. |
author_facet | Hou, Shengwei López-Pérez, Mario Pfreundt, Ulrike Belkin, Natalia Stüber, Kurt Huettel, Bruno Reinhardt, Richard Berman-Frank, Ilana Rodriguez-Valera, Francisco Hess, Wolfgang R. |
author_sort | Hou, Shengwei |
collection | PubMed |
description | Interactions between co-existing microorganisms deeply affect the physiology of the involved organisms and, ultimately, the function of the ecosystem as a whole. Copiotrophic Alteromonas are marine gammaproteobacteria that thrive during the late stages of phytoplankton blooms in the marine environment and in laboratory co-cultures with cyanobacteria such as Trichodesmium. The response of this heterotroph to the sometimes rapid and transient changes in nutrient supply when the phototroph crashes is not well understood. Here, we isolated and sequenced the strain Alteromonas macleodii str. Te101 from a laboratory culture of Trichodesmium erythraeum IMS101, yielding a chromosome of 4.63 Mb and a single plasmid of 237 kb. Increasing salinities to ≥43 ppt inhibited the growth of Trichodesmium but stimulated growth of the associated Alteromonas. We characterized the transcriptomic responses of both microorganisms and identified the complement of active transcriptional start sites in Alteromonas at single-nucleotide resolution. In replicate cultures, a similar set of genes became activated in Alteromonas when growth rates of Trichodesmium declined and mortality was high. The parallel activation of fliA, rpoS and of flagellar assembly and growth-related genes indicated that Alteromonas might have increased cell motility, growth, and multiple biosynthetic activities. Genes with the highest expression in the data set were three small RNAs (Aln1a-c) that were identified as analogs of the small RNAs CsrB-C in E. coli or RsmX-Z in pathogenic bacteria. Together with the carbon storage protein A (CsrA) homolog Te101_05290, these RNAs likely control the expression of numerous genes in responding to changes in the environment. |
format | Online Article Text |
id | pubmed-5864184 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58641842018-06-20 Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise Hou, Shengwei López-Pérez, Mario Pfreundt, Ulrike Belkin, Natalia Stüber, Kurt Huettel, Bruno Reinhardt, Richard Berman-Frank, Ilana Rodriguez-Valera, Francisco Hess, Wolfgang R. ISME J Article Interactions between co-existing microorganisms deeply affect the physiology of the involved organisms and, ultimately, the function of the ecosystem as a whole. Copiotrophic Alteromonas are marine gammaproteobacteria that thrive during the late stages of phytoplankton blooms in the marine environment and in laboratory co-cultures with cyanobacteria such as Trichodesmium. The response of this heterotroph to the sometimes rapid and transient changes in nutrient supply when the phototroph crashes is not well understood. Here, we isolated and sequenced the strain Alteromonas macleodii str. Te101 from a laboratory culture of Trichodesmium erythraeum IMS101, yielding a chromosome of 4.63 Mb and a single plasmid of 237 kb. Increasing salinities to ≥43 ppt inhibited the growth of Trichodesmium but stimulated growth of the associated Alteromonas. We characterized the transcriptomic responses of both microorganisms and identified the complement of active transcriptional start sites in Alteromonas at single-nucleotide resolution. In replicate cultures, a similar set of genes became activated in Alteromonas when growth rates of Trichodesmium declined and mortality was high. The parallel activation of fliA, rpoS and of flagellar assembly and growth-related genes indicated that Alteromonas might have increased cell motility, growth, and multiple biosynthetic activities. Genes with the highest expression in the data set were three small RNAs (Aln1a-c) that were identified as analogs of the small RNAs CsrB-C in E. coli or RsmX-Z in pathogenic bacteria. Together with the carbon storage protein A (CsrA) homolog Te101_05290, these RNAs likely control the expression of numerous genes in responding to changes in the environment. Nature Publishing Group UK 2018-01-15 2018-04 /pmc/articles/PMC5864184/ /pubmed/29335641 http://dx.doi.org/10.1038/s41396-017-0034-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hou, Shengwei López-Pérez, Mario Pfreundt, Ulrike Belkin, Natalia Stüber, Kurt Huettel, Bruno Reinhardt, Richard Berman-Frank, Ilana Rodriguez-Valera, Francisco Hess, Wolfgang R. Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise |
title | Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise |
title_full | Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise |
title_fullStr | Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise |
title_full_unstemmed | Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise |
title_short | Benefit from decline: the primary transcriptome of Alteromonas macleodii str. Te101 during Trichodesmium demise |
title_sort | benefit from decline: the primary transcriptome of alteromonas macleodii str. te101 during trichodesmium demise |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864184/ https://www.ncbi.nlm.nih.gov/pubmed/29335641 http://dx.doi.org/10.1038/s41396-017-0034-4 |
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