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The spatial and metabolic basis of colony size variation

Spatial structure impacts microbial growth and interactions, with ecological and evolutionary consequences. It is therefore important to quantitatively understand how spatial proximity affects interactions in different environments. We tested how proximity influences colony size when either Escheric...

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Autores principales: Chacón, Jeremy M., Möbius, Wolfram, Harcombe, William R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864198/
https://www.ncbi.nlm.nih.gov/pubmed/29367665
http://dx.doi.org/10.1038/s41396-017-0038-0
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author Chacón, Jeremy M.
Möbius, Wolfram
Harcombe, William R.
author_facet Chacón, Jeremy M.
Möbius, Wolfram
Harcombe, William R.
author_sort Chacón, Jeremy M.
collection PubMed
description Spatial structure impacts microbial growth and interactions, with ecological and evolutionary consequences. It is therefore important to quantitatively understand how spatial proximity affects interactions in different environments. We tested how proximity influences colony size when either Escherichia coli or Salmonella enterica are grown on various carbon sources. The importance of colony location changed with species and carbon source. Spatially explicit, genome-scale metabolic modeling recapitulated observed colony size variation. Competitors that determine territory size, according to Voronoi diagrams, were the most important drivers of variation in colony size. However, the relative importance of different competitors changed through time. Further, the effect of location increased when colonies took up resources quickly relative to the diffusion of limiting resources. These analyses made it apparent that the importance of location was smaller than expected for experiments with S. enterica growing on glucose. The accumulation of toxic byproducts appeared to limit the growth of large colonies and reduced variation in colony size. Our work provides an experimentally and theoretically grounded understanding of how location interacts with metabolism and diffusion to influence microbial interactions.
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spelling pubmed-58641982018-06-20 The spatial and metabolic basis of colony size variation Chacón, Jeremy M. Möbius, Wolfram Harcombe, William R. ISME J Article Spatial structure impacts microbial growth and interactions, with ecological and evolutionary consequences. It is therefore important to quantitatively understand how spatial proximity affects interactions in different environments. We tested how proximity influences colony size when either Escherichia coli or Salmonella enterica are grown on various carbon sources. The importance of colony location changed with species and carbon source. Spatially explicit, genome-scale metabolic modeling recapitulated observed colony size variation. Competitors that determine territory size, according to Voronoi diagrams, were the most important drivers of variation in colony size. However, the relative importance of different competitors changed through time. Further, the effect of location increased when colonies took up resources quickly relative to the diffusion of limiting resources. These analyses made it apparent that the importance of location was smaller than expected for experiments with S. enterica growing on glucose. The accumulation of toxic byproducts appeared to limit the growth of large colonies and reduced variation in colony size. Our work provides an experimentally and theoretically grounded understanding of how location interacts with metabolism and diffusion to influence microbial interactions. Nature Publishing Group UK 2018-01-24 2018-03 /pmc/articles/PMC5864198/ /pubmed/29367665 http://dx.doi.org/10.1038/s41396-017-0038-0 Text en © International Society for Microbial Ecology 2018 Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.
spellingShingle Article
Chacón, Jeremy M.
Möbius, Wolfram
Harcombe, William R.
The spatial and metabolic basis of colony size variation
title The spatial and metabolic basis of colony size variation
title_full The spatial and metabolic basis of colony size variation
title_fullStr The spatial and metabolic basis of colony size variation
title_full_unstemmed The spatial and metabolic basis of colony size variation
title_short The spatial and metabolic basis of colony size variation
title_sort spatial and metabolic basis of colony size variation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864198/
https://www.ncbi.nlm.nih.gov/pubmed/29367665
http://dx.doi.org/10.1038/s41396-017-0038-0
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