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Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole
OBJECTIVE: Breast cancer is the most common cancer in women worldwide and the incidence increases in postmenopausal women. Anastrozole is a non-steroidal (type II), third-generation aromatase inhibitor (AI) that is used in the treatment of postmenopausal estrogen-related breast cancer. Several studi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kare Publishing
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864710/ https://www.ncbi.nlm.nih.gov/pubmed/29607425 http://dx.doi.org/10.14744/nci.2017.55822 |
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author | Tugce Yesil, Devecioglu Aydogan, Fatih Omurtag, Gulden Zehra Bese, Nuran Senel Sardas, Semra |
author_facet | Tugce Yesil, Devecioglu Aydogan, Fatih Omurtag, Gulden Zehra Bese, Nuran Senel Sardas, Semra |
author_sort | Tugce Yesil, Devecioglu |
collection | PubMed |
description | OBJECTIVE: Breast cancer is the most common cancer in women worldwide and the incidence increases in postmenopausal women. Anastrozole is a non-steroidal (type II), third-generation aromatase inhibitor (AI) that is used in the treatment of postmenopausal estrogen-related breast cancer. Several studies have been conducted to assess the efficacy, safety, and superiority of AIs to tamoxifen; however, a literature search did not reveal a study that investigated the genotoxic potential of AIs. The aim of this study was to investigate the possible DNA damage risk profile and individual DNA repair capacity of patients using anastrozole with the modified alkaline comet assay in order to contribute to public health and health economics. METHODS: Women diagnosed with breast cancer after menopause comprised the study group. Six patients who had taken anastrozole for at least 6 months were retrospectively enrolled, and 12 patients who had not yet received treatment were prospectively enrolled as a control group. Peripheral blood lymphocytes were used to measure oxidized DNA damage using formamidopyrimidine DNA-glycosylase (FPG) and endonuclease III (endo III) in a modified comet assay. Individual DNA repair capacity was evaluated with the comet assay after a hydrogen peroxide (H(2)O(2)) challenge to examine the difference in DNA damage susceptibility. RESULTS: Analysis of DNA damage, oxidative base damage, susceptibility to DNA damage, and repair capacity revealed no significant difference between the control group and the patients taking anastrozole (p>0.05). Susceptibility to H(2)O(2) damage was observed to increase with age (p<0.05). CONCLUSION: According to the results obtained in this study, anastrozole did not contribute to oxidative DNA damage. An H(2)O(2) challenge with the comet assay is useful to evaluate circumstances of increased vulnerability to damage, such as aging and cancer. |
format | Online Article Text |
id | pubmed-5864710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Kare Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-58647102018-03-30 Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole Tugce Yesil, Devecioglu Aydogan, Fatih Omurtag, Gulden Zehra Bese, Nuran Senel Sardas, Semra North Clin Istanb Original Article OBJECTIVE: Breast cancer is the most common cancer in women worldwide and the incidence increases in postmenopausal women. Anastrozole is a non-steroidal (type II), third-generation aromatase inhibitor (AI) that is used in the treatment of postmenopausal estrogen-related breast cancer. Several studies have been conducted to assess the efficacy, safety, and superiority of AIs to tamoxifen; however, a literature search did not reveal a study that investigated the genotoxic potential of AIs. The aim of this study was to investigate the possible DNA damage risk profile and individual DNA repair capacity of patients using anastrozole with the modified alkaline comet assay in order to contribute to public health and health economics. METHODS: Women diagnosed with breast cancer after menopause comprised the study group. Six patients who had taken anastrozole for at least 6 months were retrospectively enrolled, and 12 patients who had not yet received treatment were prospectively enrolled as a control group. Peripheral blood lymphocytes were used to measure oxidized DNA damage using formamidopyrimidine DNA-glycosylase (FPG) and endonuclease III (endo III) in a modified comet assay. Individual DNA repair capacity was evaluated with the comet assay after a hydrogen peroxide (H(2)O(2)) challenge to examine the difference in DNA damage susceptibility. RESULTS: Analysis of DNA damage, oxidative base damage, susceptibility to DNA damage, and repair capacity revealed no significant difference between the control group and the patients taking anastrozole (p>0.05). Susceptibility to H(2)O(2) damage was observed to increase with age (p<0.05). CONCLUSION: According to the results obtained in this study, anastrozole did not contribute to oxidative DNA damage. An H(2)O(2) challenge with the comet assay is useful to evaluate circumstances of increased vulnerability to damage, such as aging and cancer. Kare Publishing 2018-01-22 /pmc/articles/PMC5864710/ /pubmed/29607425 http://dx.doi.org/10.14744/nci.2017.55822 Text en Copyright: © 2018 by Istanbul Northern Anatolian Association of Public Hospitals http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License |
spellingShingle | Original Article Tugce Yesil, Devecioglu Aydogan, Fatih Omurtag, Gulden Zehra Bese, Nuran Senel Sardas, Semra Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole |
title | Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole |
title_full | Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole |
title_fullStr | Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole |
title_full_unstemmed | Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole |
title_short | Investigation of genotoxicity risk and DNA repair capacity in breast cancer patients using anastrozole |
title_sort | investigation of genotoxicity risk and dna repair capacity in breast cancer patients using anastrozole |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864710/ https://www.ncbi.nlm.nih.gov/pubmed/29607425 http://dx.doi.org/10.14744/nci.2017.55822 |
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