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Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly

Low bone mineral density (BMD) is a risk factor of osteoporotic fracture (OF). Peripheral blood monocytes (PBM) can differentiate into osteoclasts to resorb bone. It was known that PBM-expressed Anxa2 protein is associated with BMD, and extracellular Anxa2 protein promotes osteoclastogenesis. This s...

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Autores principales: Zhou, Xu, Wu, Long-Fei, Wang, Wen-Yu, Lu, Xin, Jiang, Zhen-Huan, Zhang, Yun-Hong, Jiang, Ding-Hua, Jiang, Jian-Nong, Gao, Hong-Qing, Lei, Shu-Feng, Deng, Fei-Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865729/
https://www.ncbi.nlm.nih.gov/pubmed/29570731
http://dx.doi.org/10.1371/journal.pone.0194781
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author Zhou, Xu
Wu, Long-Fei
Wang, Wen-Yu
Lu, Xin
Jiang, Zhen-Huan
Zhang, Yun-Hong
Jiang, Ding-Hua
Jiang, Jian-Nong
Gao, Hong-Qing
Lei, Shu-Feng
Deng, Fei-Yan
author_facet Zhou, Xu
Wu, Long-Fei
Wang, Wen-Yu
Lu, Xin
Jiang, Zhen-Huan
Zhang, Yun-Hong
Jiang, Ding-Hua
Jiang, Jian-Nong
Gao, Hong-Qing
Lei, Shu-Feng
Deng, Fei-Yan
author_sort Zhou, Xu
collection PubMed
description Low bone mineral density (BMD) is a risk factor of osteoporotic fracture (OF). Peripheral blood monocytes (PBM) can differentiate into osteoclasts to resorb bone. It was known that PBM-expressed Anxa2 protein is associated with BMD, and extracellular Anxa2 protein promotes osteoclastogenesis. This study aimed to test 1) whether Anxa2 protein level in PBM differs significantly between subjects with OF and without fracture history (NF); 2) whether Anxa2 level in plasma is associated with BMD; 3) how Anxa2 protein at various concentrations would affect osteoblastic activity in vitro. All the study subjects were Chinese Han elderly. Firstly, Anxa2 protein in PBM was identified and quantitated by LC-MS/MS and compared between 45 OF cases and 42 healthy controls. Secondly, plasma Anxa2 protein level was quantitated by ELISA and compared between unrelated subjects with extremely low vs. high hip BMD (0.63±0.10 vs. 1.05±0.10 g/cm(2), n = 75). Furthermore, in vitro functional assay was utilized to test the effects of extracellular Anxa2 protein on osteoblastic growth. We found that Anxa2 protein expression in PBM was significantly up-regulated in OF vs. NF subjects (fold change [FC)] = 1.16, P<0.05). Plasma Anxa2 protein concentration (range: 31.69–227.35ng/ml) was significantly elevated in low vs. high BMD subjects (84.85 vs. 66.15ng/ml, FC = 1.28, P<0.05). Cellular dynamical monitoring demonstrated that the general shape of dose-response relationship is the inverse U-shaped curve. Specifically, lower dose of Anxa2 protein may promote osteoblast growth and the optimal concentration for osteoblastic growth was around 50ng/ml, but even higher concentration could attenuate hFOB1.19 osteoprogenitor cell growth. We concluded that Anxa2 protein could attenuate osteoblast growth and be associated with hip BMD and OF in Chinese elderly.
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spelling pubmed-58657292018-03-28 Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly Zhou, Xu Wu, Long-Fei Wang, Wen-Yu Lu, Xin Jiang, Zhen-Huan Zhang, Yun-Hong Jiang, Ding-Hua Jiang, Jian-Nong Gao, Hong-Qing Lei, Shu-Feng Deng, Fei-Yan PLoS One Research Article Low bone mineral density (BMD) is a risk factor of osteoporotic fracture (OF). Peripheral blood monocytes (PBM) can differentiate into osteoclasts to resorb bone. It was known that PBM-expressed Anxa2 protein is associated with BMD, and extracellular Anxa2 protein promotes osteoclastogenesis. This study aimed to test 1) whether Anxa2 protein level in PBM differs significantly between subjects with OF and without fracture history (NF); 2) whether Anxa2 level in plasma is associated with BMD; 3) how Anxa2 protein at various concentrations would affect osteoblastic activity in vitro. All the study subjects were Chinese Han elderly. Firstly, Anxa2 protein in PBM was identified and quantitated by LC-MS/MS and compared between 45 OF cases and 42 healthy controls. Secondly, plasma Anxa2 protein level was quantitated by ELISA and compared between unrelated subjects with extremely low vs. high hip BMD (0.63±0.10 vs. 1.05±0.10 g/cm(2), n = 75). Furthermore, in vitro functional assay was utilized to test the effects of extracellular Anxa2 protein on osteoblastic growth. We found that Anxa2 protein expression in PBM was significantly up-regulated in OF vs. NF subjects (fold change [FC)] = 1.16, P<0.05). Plasma Anxa2 protein concentration (range: 31.69–227.35ng/ml) was significantly elevated in low vs. high BMD subjects (84.85 vs. 66.15ng/ml, FC = 1.28, P<0.05). Cellular dynamical monitoring demonstrated that the general shape of dose-response relationship is the inverse U-shaped curve. Specifically, lower dose of Anxa2 protein may promote osteoblast growth and the optimal concentration for osteoblastic growth was around 50ng/ml, but even higher concentration could attenuate hFOB1.19 osteoprogenitor cell growth. We concluded that Anxa2 protein could attenuate osteoblast growth and be associated with hip BMD and OF in Chinese elderly. Public Library of Science 2018-03-23 /pmc/articles/PMC5865729/ /pubmed/29570731 http://dx.doi.org/10.1371/journal.pone.0194781 Text en © 2018 Zhou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhou, Xu
Wu, Long-Fei
Wang, Wen-Yu
Lu, Xin
Jiang, Zhen-Huan
Zhang, Yun-Hong
Jiang, Ding-Hua
Jiang, Jian-Nong
Gao, Hong-Qing
Lei, Shu-Feng
Deng, Fei-Yan
Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly
title Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly
title_full Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly
title_fullStr Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly
title_full_unstemmed Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly
title_short Anxa2 attenuates osteoblast growth and is associated with hip BMD and osteoporotic fracture in Chinese elderly
title_sort anxa2 attenuates osteoblast growth and is associated with hip bmd and osteoporotic fracture in chinese elderly
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865729/
https://www.ncbi.nlm.nih.gov/pubmed/29570731
http://dx.doi.org/10.1371/journal.pone.0194781
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