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MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression
Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR-1288 remains poorly understood, and its role in GBM has not been reported. The present study confirmed that miR-...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865833/ https://www.ncbi.nlm.nih.gov/pubmed/28901464 http://dx.doi.org/10.3892/mmr.2017.7481 |
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author | Yin, Jun Weng, Chengyin Ma, Jieke Chen, Fanfan Huang, Yecai Feng, Mei |
author_facet | Yin, Jun Weng, Chengyin Ma, Jieke Chen, Fanfan Huang, Yecai Feng, Mei |
author_sort | Yin, Jun |
collection | PubMed |
description | Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR-1288 remains poorly understood, and its role in GBM has not been reported. The present study confirmed that miR-1288 expression was markedly upregulated in GBM. Ectopic expression of miR-1288 promoted the proliferation, colony formation and anchorage-independent growth of GBM cells. Bioinformatics analysis coupled with western blotting and luciferase report assays also indicated that miR-1288 promoted cell proliferation of GBM by targeting ubiquitin carboxyl-terminal hydrolase (CYLD). Knockdown of CYLD expression reversed the cell proliferation promotion by miR-1288-in in GBM. These results suggest that the miR-1288/CYLD axis may represent a potential therapeutic target for the treatment of GBM. |
format | Online Article Text |
id | pubmed-5865833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-58658332018-03-27 MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression Yin, Jun Weng, Chengyin Ma, Jieke Chen, Fanfan Huang, Yecai Feng, Mei Mol Med Rep Articles Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR-1288 remains poorly understood, and its role in GBM has not been reported. The present study confirmed that miR-1288 expression was markedly upregulated in GBM. Ectopic expression of miR-1288 promoted the proliferation, colony formation and anchorage-independent growth of GBM cells. Bioinformatics analysis coupled with western blotting and luciferase report assays also indicated that miR-1288 promoted cell proliferation of GBM by targeting ubiquitin carboxyl-terminal hydrolase (CYLD). Knockdown of CYLD expression reversed the cell proliferation promotion by miR-1288-in in GBM. These results suggest that the miR-1288/CYLD axis may represent a potential therapeutic target for the treatment of GBM. D.A. Spandidos 2017-11 2017-09-13 /pmc/articles/PMC5865833/ /pubmed/28901464 http://dx.doi.org/10.3892/mmr.2017.7481 Text en Copyright: © Yin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yin, Jun Weng, Chengyin Ma, Jieke Chen, Fanfan Huang, Yecai Feng, Mei MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression |
title | MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression |
title_full | MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression |
title_fullStr | MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression |
title_full_unstemmed | MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression |
title_short | MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression |
title_sort | microrna-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase cyld expression |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865833/ https://www.ncbi.nlm.nih.gov/pubmed/28901464 http://dx.doi.org/10.3892/mmr.2017.7481 |
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