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MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression

Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR-1288 remains poorly understood, and its role in GBM has not been reported. The present study confirmed that miR-...

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Detalles Bibliográficos
Autores principales: Yin, Jun, Weng, Chengyin, Ma, Jieke, Chen, Fanfan, Huang, Yecai, Feng, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865833/
https://www.ncbi.nlm.nih.gov/pubmed/28901464
http://dx.doi.org/10.3892/mmr.2017.7481
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author Yin, Jun
Weng, Chengyin
Ma, Jieke
Chen, Fanfan
Huang, Yecai
Feng, Mei
author_facet Yin, Jun
Weng, Chengyin
Ma, Jieke
Chen, Fanfan
Huang, Yecai
Feng, Mei
author_sort Yin, Jun
collection PubMed
description Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR-1288 remains poorly understood, and its role in GBM has not been reported. The present study confirmed that miR-1288 expression was markedly upregulated in GBM. Ectopic expression of miR-1288 promoted the proliferation, colony formation and anchorage-independent growth of GBM cells. Bioinformatics analysis coupled with western blotting and luciferase report assays also indicated that miR-1288 promoted cell proliferation of GBM by targeting ubiquitin carboxyl-terminal hydrolase (CYLD). Knockdown of CYLD expression reversed the cell proliferation promotion by miR-1288-in in GBM. These results suggest that the miR-1288/CYLD axis may represent a potential therapeutic target for the treatment of GBM.
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spelling pubmed-58658332018-03-27 MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression Yin, Jun Weng, Chengyin Ma, Jieke Chen, Fanfan Huang, Yecai Feng, Mei Mol Med Rep Articles Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR-1288 remains poorly understood, and its role in GBM has not been reported. The present study confirmed that miR-1288 expression was markedly upregulated in GBM. Ectopic expression of miR-1288 promoted the proliferation, colony formation and anchorage-independent growth of GBM cells. Bioinformatics analysis coupled with western blotting and luciferase report assays also indicated that miR-1288 promoted cell proliferation of GBM by targeting ubiquitin carboxyl-terminal hydrolase (CYLD). Knockdown of CYLD expression reversed the cell proliferation promotion by miR-1288-in in GBM. These results suggest that the miR-1288/CYLD axis may represent a potential therapeutic target for the treatment of GBM. D.A. Spandidos 2017-11 2017-09-13 /pmc/articles/PMC5865833/ /pubmed/28901464 http://dx.doi.org/10.3892/mmr.2017.7481 Text en Copyright: © Yin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yin, Jun
Weng, Chengyin
Ma, Jieke
Chen, Fanfan
Huang, Yecai
Feng, Mei
MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression
title MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression
title_full MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression
title_fullStr MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression
title_full_unstemmed MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression
title_short MicroRNA-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase CYLD expression
title_sort microrna-1288 promotes cell proliferation of human glioblastoma cells by repressing ubiquitin carboxyl-terminal hydrolase cyld expression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865833/
https://www.ncbi.nlm.nih.gov/pubmed/28901464
http://dx.doi.org/10.3892/mmr.2017.7481
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