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Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1

The formation of fibrocartilage at the healing site following a rotator cuff tear repair is a major problem in the field of tendon-bone healing. The present study aimed to enhance the healing of the tendon-bone interface following rotator-cuff tear repair by the interposition of hydroxyapatite (HA)...

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Detalles Bibliográficos
Autores principales: You, Xiebo, Shen, Yueqin, Yu, Weihan, He, Yaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865958/
https://www.ncbi.nlm.nih.gov/pubmed/29393349
http://dx.doi.org/10.3892/mmr.2018.8499
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author You, Xiebo
Shen, Yueqin
Yu, Weihan
He, Yaohua
author_facet You, Xiebo
Shen, Yueqin
Yu, Weihan
He, Yaohua
author_sort You, Xiebo
collection PubMed
description The formation of fibrocartilage at the healing site following a rotator cuff tear repair is a major problem in the field of tendon-bone healing. The present study aimed to enhance the healing of the tendon-bone interface following rotator-cuff tear repair by the interposition of hydroxyapatite (HA) encapsulated with transforming growth factor β1 (TGFβ1). Using an acute rotator cuff repair model, rats were divided into three groups: i) Repair only (control); ii) HA group; and iii) HA-TGFβ1 group. Animals were sacrificed at 2, 4 and 8 weeks following surgery. Micro-computed tomography (CT), histomorphometric analyses and biomechanical tests were used to evaluate the supraspinatus tendon-bone complex. The micro-CT images revealed notable novel bone formation in the groups treated with HA-TGFβ1. The histomorphometric analyses demonstrated improved fibrocartilage formation and collagen organization at the tendon-bone interface. The HA-TGFβ1 combination significantly improved the area of fibrocartilage, particularly at early time points (2 and 4 weeks). There was a significantly greater load-to-failure force achieved in the HA and HA-TGFβ1 groups compared with the control group at 4 and 8 weeks. Augmentation of the ceramic powder with HA-TGFβ1 at the tendon-bone interface was demonstrated to strengthen the healing entheses, increase bone and fibrocartilage formation and improve collagen organization compared with surgical repair alone. Local application of HA-TGFβ1 demonstrates potential in improving tendon-bone healing following rotator cuff repair.
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spelling pubmed-58659582018-03-28 Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1 You, Xiebo Shen, Yueqin Yu, Weihan He, Yaohua Mol Med Rep Articles The formation of fibrocartilage at the healing site following a rotator cuff tear repair is a major problem in the field of tendon-bone healing. The present study aimed to enhance the healing of the tendon-bone interface following rotator-cuff tear repair by the interposition of hydroxyapatite (HA) encapsulated with transforming growth factor β1 (TGFβ1). Using an acute rotator cuff repair model, rats were divided into three groups: i) Repair only (control); ii) HA group; and iii) HA-TGFβ1 group. Animals were sacrificed at 2, 4 and 8 weeks following surgery. Micro-computed tomography (CT), histomorphometric analyses and biomechanical tests were used to evaluate the supraspinatus tendon-bone complex. The micro-CT images revealed notable novel bone formation in the groups treated with HA-TGFβ1. The histomorphometric analyses demonstrated improved fibrocartilage formation and collagen organization at the tendon-bone interface. The HA-TGFβ1 combination significantly improved the area of fibrocartilage, particularly at early time points (2 and 4 weeks). There was a significantly greater load-to-failure force achieved in the HA and HA-TGFβ1 groups compared with the control group at 4 and 8 weeks. Augmentation of the ceramic powder with HA-TGFβ1 at the tendon-bone interface was demonstrated to strengthen the healing entheses, increase bone and fibrocartilage formation and improve collagen organization compared with surgical repair alone. Local application of HA-TGFβ1 demonstrates potential in improving tendon-bone healing following rotator cuff repair. D.A. Spandidos 2018-04 2018-01-26 /pmc/articles/PMC5865958/ /pubmed/29393349 http://dx.doi.org/10.3892/mmr.2018.8499 Text en Copyright: © You et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
You, Xiebo
Shen, Yueqin
Yu, Weihan
He, Yaohua
Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1
title Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1
title_full Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1
title_fullStr Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1
title_full_unstemmed Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1
title_short Enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with TGFβ1
title_sort enhancement of tendon-bone healing following rotator cuff repair using hydroxyapatite with tgfβ1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865958/
https://www.ncbi.nlm.nih.gov/pubmed/29393349
http://dx.doi.org/10.3892/mmr.2018.8499
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