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Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion

IgA nephropathy (IgAN) is characterized by predominant IgA deposition in the glomerular mesangium. It has been considered that the deposited IgA is synthesized by B cells, although recent reports have suggested the implication of other cell types. Therefore, the present study investigated whether gl...

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Autores principales: Deng, Hui, Ma, Junfan, Jing, Ziyang, Deng, Zhenling, Liang, Yaoxian, A, LATA, Liu, Yang, Qiu, Xiaoyan, Wang, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865995/
https://www.ncbi.nlm.nih.gov/pubmed/29393471
http://dx.doi.org/10.3892/mmr.2018.8544
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author Deng, Hui
Ma, Junfan
Jing, Ziyang
Deng, Zhenling
Liang, Yaoxian
A, LATA
Liu, Yang
Qiu, Xiaoyan
Wang, Yue
author_facet Deng, Hui
Ma, Junfan
Jing, Ziyang
Deng, Zhenling
Liang, Yaoxian
A, LATA
Liu, Yang
Qiu, Xiaoyan
Wang, Yue
author_sort Deng, Hui
collection PubMed
description IgA nephropathy (IgAN) is characterized by predominant IgA deposition in the glomerular mesangium. It has been considered that the deposited IgA is synthesized by B cells, although recent reports have suggested the implication of other cell types. Therefore, the present study investigated whether glomerular mesangial cells could produce IgA by themselves. Semi-quantitative reverse transcription-polymerase chain reaction, and immunostaining analysis revealed that the IgA protein and gene transcripts were expressed in primary human renal mesangial cells (HRMCs). Furthermore, the IgA heavy chain (α1 and α2) and the light chain (κ and λ) were localized in the cytoplasm or were located on the cell membranes of human mesangial cells (HMCs). Mass spectrometry results indicated that Ig α1 and Ig α2 were secreted in the culture media of HMCs. The transcripts of Ig α, Ig κ and Ig λ constant regions were detected. The predominant rearrangement pattern of the variable region of Ig κ, was Vκ3-20*01/Jκ1*01 in HMCs and Vκ1-12*01/Jκ4*01 in HRMCs. In addition, knockdown of Ig α1 expression by small interfering RNA (siRNA) inhibited cell adhesion and promoted apoptosis. Our findings demonstrate that HMCs can express IgA, and that this expression is associated with cell functions, which may contribute to the deposition of IgA in patients with IgAN.
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spelling pubmed-58659952018-03-28 Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion Deng, Hui Ma, Junfan Jing, Ziyang Deng, Zhenling Liang, Yaoxian A, LATA Liu, Yang Qiu, Xiaoyan Wang, Yue Mol Med Rep Articles IgA nephropathy (IgAN) is characterized by predominant IgA deposition in the glomerular mesangium. It has been considered that the deposited IgA is synthesized by B cells, although recent reports have suggested the implication of other cell types. Therefore, the present study investigated whether glomerular mesangial cells could produce IgA by themselves. Semi-quantitative reverse transcription-polymerase chain reaction, and immunostaining analysis revealed that the IgA protein and gene transcripts were expressed in primary human renal mesangial cells (HRMCs). Furthermore, the IgA heavy chain (α1 and α2) and the light chain (κ and λ) were localized in the cytoplasm or were located on the cell membranes of human mesangial cells (HMCs). Mass spectrometry results indicated that Ig α1 and Ig α2 were secreted in the culture media of HMCs. The transcripts of Ig α, Ig κ and Ig λ constant regions were detected. The predominant rearrangement pattern of the variable region of Ig κ, was Vκ3-20*01/Jκ1*01 in HMCs and Vκ1-12*01/Jκ4*01 in HRMCs. In addition, knockdown of Ig α1 expression by small interfering RNA (siRNA) inhibited cell adhesion and promoted apoptosis. Our findings demonstrate that HMCs can express IgA, and that this expression is associated with cell functions, which may contribute to the deposition of IgA in patients with IgAN. D.A. Spandidos 2018-04 2018-02-02 /pmc/articles/PMC5865995/ /pubmed/29393471 http://dx.doi.org/10.3892/mmr.2018.8544 Text en Copyright: © Deng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Deng, Hui
Ma, Junfan
Jing, Ziyang
Deng, Zhenling
Liang, Yaoxian
A, LATA
Liu, Yang
Qiu, Xiaoyan
Wang, Yue
Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion
title Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion
title_full Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion
title_fullStr Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion
title_full_unstemmed Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion
title_short Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion
title_sort expression of immunoglobulin a in human mesangial cells and its effects on cell apoptosis and adhesion
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5865995/
https://www.ncbi.nlm.nih.gov/pubmed/29393471
http://dx.doi.org/10.3892/mmr.2018.8544
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