Cargando…
Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells
The actions of thyroid hormone (TH) on lipid metabolism in the liver are associated with a number of genes involved in lipogenesis and lipid metabolism; however, the underlying mechanisms through which TH impacts on lipid metabolism remain to be elucidated. The present study aimed to investigate the...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866004/ https://www.ncbi.nlm.nih.gov/pubmed/29484422 http://dx.doi.org/10.3892/mmr.2018.8633 |
_version_ | 1783308791804067840 |
---|---|
author | Zheng, Yingjuan Zhao, Chao Zhang, Naijian Kang, Wenqin Lu, Rongrong Wu, Huadong Geng, Yingxue Zhao, Yaping Xu, Xiaoyan |
author_facet | Zheng, Yingjuan Zhao, Chao Zhang, Naijian Kang, Wenqin Lu, Rongrong Wu, Huadong Geng, Yingxue Zhao, Yaping Xu, Xiaoyan |
author_sort | Zheng, Yingjuan |
collection | PubMed |
description | The actions of thyroid hormone (TH) on lipid metabolism in the liver are associated with a number of genes involved in lipogenesis and lipid metabolism; however, the underlying mechanisms through which TH impacts on lipid metabolism remain to be elucidated. The present study aimed to investigate the effects of hyperthyroidism on the serum levels of the microRNA (miR) miR-206 and the role of miR-206 on TH-regulated lipid metabolism in liver cells. Serum was obtained from 12 patients diagnosed with hyperthyroidism and 10 healthy control subjects. Human hepatoblastoma (HepG2) cells were used to study the effects of triiodothyronine (T3) and miR-206 on lipid metabolism. Expression of miR-206 in serum and cells was determined by reverse transcription-quantitative polymerase chain reaction analysis. Lipid accumulation in HepG2 cells was assessed with Oil Red O staining. Suppression or overexpression of miR-206 was performed via transfection with a miR-206 mimic or miR-206 inhibitor. Serum miR-206 was significantly decreased in patients with hyperthyroidism compared with euthyroid controls. Treatment of HepG2 cells with T3 led to reduced total cholesterol (TC) and triglyceride (TG) content, accompanied by reduced miR-206 expression. Inhibition of endogenous miR-206 expression decreased intracellular TG and TC content in HepG2 cells. By contrast, overexpression of miR-206 in HepG2 partially prevented the reduction in TG content induced by treatment with T3. In conclusion, serum miR-206 expression is reduced in patients with hyperthyroidism. In addition, miR-206 is involved in T3-mediated regulation of lipid metabolism in HepG2 cells, indicating a role for miR-206 in thyroid hormone-induced disorders of lipid metabolism in the liver. |
format | Online Article Text |
id | pubmed-5866004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-58660042018-03-28 Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells Zheng, Yingjuan Zhao, Chao Zhang, Naijian Kang, Wenqin Lu, Rongrong Wu, Huadong Geng, Yingxue Zhao, Yaping Xu, Xiaoyan Mol Med Rep Articles The actions of thyroid hormone (TH) on lipid metabolism in the liver are associated with a number of genes involved in lipogenesis and lipid metabolism; however, the underlying mechanisms through which TH impacts on lipid metabolism remain to be elucidated. The present study aimed to investigate the effects of hyperthyroidism on the serum levels of the microRNA (miR) miR-206 and the role of miR-206 on TH-regulated lipid metabolism in liver cells. Serum was obtained from 12 patients diagnosed with hyperthyroidism and 10 healthy control subjects. Human hepatoblastoma (HepG2) cells were used to study the effects of triiodothyronine (T3) and miR-206 on lipid metabolism. Expression of miR-206 in serum and cells was determined by reverse transcription-quantitative polymerase chain reaction analysis. Lipid accumulation in HepG2 cells was assessed with Oil Red O staining. Suppression or overexpression of miR-206 was performed via transfection with a miR-206 mimic or miR-206 inhibitor. Serum miR-206 was significantly decreased in patients with hyperthyroidism compared with euthyroid controls. Treatment of HepG2 cells with T3 led to reduced total cholesterol (TC) and triglyceride (TG) content, accompanied by reduced miR-206 expression. Inhibition of endogenous miR-206 expression decreased intracellular TG and TC content in HepG2 cells. By contrast, overexpression of miR-206 in HepG2 partially prevented the reduction in TG content induced by treatment with T3. In conclusion, serum miR-206 expression is reduced in patients with hyperthyroidism. In addition, miR-206 is involved in T3-mediated regulation of lipid metabolism in HepG2 cells, indicating a role for miR-206 in thyroid hormone-induced disorders of lipid metabolism in the liver. D.A. Spandidos 2018-04 2018-02-26 /pmc/articles/PMC5866004/ /pubmed/29484422 http://dx.doi.org/10.3892/mmr.2018.8633 Text en Copyright: © Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zheng, Yingjuan Zhao, Chao Zhang, Naijian Kang, Wenqin Lu, Rongrong Wu, Huadong Geng, Yingxue Zhao, Yaping Xu, Xiaoyan Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells |
title | Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells |
title_full | Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells |
title_fullStr | Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells |
title_full_unstemmed | Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells |
title_short | Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells |
title_sort | serum microrna mir-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in hepg2 human hepatoblastoma cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866004/ https://www.ncbi.nlm.nih.gov/pubmed/29484422 http://dx.doi.org/10.3892/mmr.2018.8633 |
work_keys_str_mv | AT zhengyingjuan serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT zhaochao serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT zhangnaijian serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT kangwenqin serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT lurongrong serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT wuhuadong serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT gengyingxue serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT zhaoyaping serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells AT xuxiaoyan serummicrornamir206isdecreasedinhyperthyroidismandmediatesthyroidhormoneregulationoflipidmetabolisminhepg2humanhepatoblastomacells |