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Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance

Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci...

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Autores principales: Zhou, Xiaogang, Liao, Haicheng, Chern, Mawsheng, Yin, Junjie, Chen, Yufei, Wang, Jianping, Zhu, Xiaobo, Chen, Zhixiong, Yuan, Can, Zhao, Wen, Wang, Jing, Li, Weitao, He, Min, Ma, Bingtian, Wang, Jichun, Qin, Peng, Chen, Weilan, Wang, Yuping, Liu, Jiali, Qian, Yangwen, Wang, Wenming, Wu, Xianjun, Li, Ping, Zhu, Lihuang, Li, Shigui, Ronald, Pamela C., Chen, Xuewei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866533/
https://www.ncbi.nlm.nih.gov/pubmed/29432165
http://dx.doi.org/10.1073/pnas.1705927115
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author Zhou, Xiaogang
Liao, Haicheng
Chern, Mawsheng
Yin, Junjie
Chen, Yufei
Wang, Jianping
Zhu, Xiaobo
Chen, Zhixiong
Yuan, Can
Zhao, Wen
Wang, Jing
Li, Weitao
He, Min
Ma, Bingtian
Wang, Jichun
Qin, Peng
Chen, Weilan
Wang, Yuping
Liu, Jiali
Qian, Yangwen
Wang, Wenming
Wu, Xianjun
Li, Ping
Zhu, Lihuang
Li, Shigui
Ronald, Pamela C.
Chen, Xuewei
author_facet Zhou, Xiaogang
Liao, Haicheng
Chern, Mawsheng
Yin, Junjie
Chen, Yufei
Wang, Jianping
Zhu, Xiaobo
Chen, Zhixiong
Yuan, Can
Zhao, Wen
Wang, Jing
Li, Weitao
He, Min
Ma, Bingtian
Wang, Jichun
Qin, Peng
Chen, Weilan
Wang, Yuping
Liu, Jiali
Qian, Yangwen
Wang, Wenming
Wu, Xianjun
Li, Ping
Zhu, Lihuang
Li, Shigui
Ronald, Pamela C.
Chen, Xuewei
author_sort Zhou, Xiaogang
collection PubMed
description Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits. Map-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats (TPRs)-containing protein, which binds to mRNAs of multiple OsPAL (OsPAL1–7) genes and promotes their turnover. Loss of function of the Bsr-k1 gene leads to accumulation of OsPAL1–7 mRNAs in the bsr-k1 mutant. Furthermore, overexpression of OsPAL1 in wild-type rice TP309 confers resistance to M. oryzae, supporting the role of OsPAL1. Our discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.
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spelling pubmed-58665332018-03-29 Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance Zhou, Xiaogang Liao, Haicheng Chern, Mawsheng Yin, Junjie Chen, Yufei Wang, Jianping Zhu, Xiaobo Chen, Zhixiong Yuan, Can Zhao, Wen Wang, Jing Li, Weitao He, Min Ma, Bingtian Wang, Jichun Qin, Peng Chen, Weilan Wang, Yuping Liu, Jiali Qian, Yangwen Wang, Wenming Wu, Xianjun Li, Ping Zhu, Lihuang Li, Shigui Ronald, Pamela C. Chen, Xuewei Proc Natl Acad Sci U S A Biological Sciences Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits. Map-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats (TPRs)-containing protein, which binds to mRNAs of multiple OsPAL (OsPAL1–7) genes and promotes their turnover. Loss of function of the Bsr-k1 gene leads to accumulation of OsPAL1–7 mRNAs in the bsr-k1 mutant. Furthermore, overexpression of OsPAL1 in wild-type rice TP309 confers resistance to M. oryzae, supporting the role of OsPAL1. Our discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice. National Academy of Sciences 2018-03-20 2018-02-05 /pmc/articles/PMC5866533/ /pubmed/29432165 http://dx.doi.org/10.1073/pnas.1705927115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Zhou, Xiaogang
Liao, Haicheng
Chern, Mawsheng
Yin, Junjie
Chen, Yufei
Wang, Jianping
Zhu, Xiaobo
Chen, Zhixiong
Yuan, Can
Zhao, Wen
Wang, Jing
Li, Weitao
He, Min
Ma, Bingtian
Wang, Jichun
Qin, Peng
Chen, Weilan
Wang, Yuping
Liu, Jiali
Qian, Yangwen
Wang, Wenming
Wu, Xianjun
Li, Ping
Zhu, Lihuang
Li, Shigui
Ronald, Pamela C.
Chen, Xuewei
Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance
title Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance
title_full Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance
title_fullStr Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance
title_full_unstemmed Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance
title_short Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance
title_sort loss of function of a rice tpr-domain rna-binding protein confers broad-spectrum disease resistance
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866533/
https://www.ncbi.nlm.nih.gov/pubmed/29432165
http://dx.doi.org/10.1073/pnas.1705927115
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