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MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity

Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. Although MERS-CoV infection is ubiquitous in dromedaries across Africa as well as in the Arabian Penin...

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Autores principales: Chu, Daniel K. W., Hui, Kenrie P. Y., Perera, Ranawaka A. P. M., Miguel, Eve, Niemeyer, Daniela, Zhao, Jincun, Channappanavar, Rudragouda, Dudas, Gytis, Oladipo, Jamiu O., Traoré, Amadou, Fassi-Fihri, Ouafaa, Ali, Abraham, Demissié, Getnet F., Muth, Doreen, Chan, Michael C. W., Nicholls, John M., Meyerholz, David K., Kuranga, Sulyman A., Mamo, Gezahegne, Zhou, Ziqi, So, Ray T. Y., Hemida, Maged G., Webby, Richard J., Roger, Francois, Rambaut, Andrew, Poon, Leo L. M., Perlman, Stanley, Drosten, Christian, Chevalier, Veronique, Peiris, Malik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866576/
https://www.ncbi.nlm.nih.gov/pubmed/29507189
http://dx.doi.org/10.1073/pnas.1718769115
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author Chu, Daniel K. W.
Hui, Kenrie P. Y.
Perera, Ranawaka A. P. M.
Miguel, Eve
Niemeyer, Daniela
Zhao, Jincun
Channappanavar, Rudragouda
Dudas, Gytis
Oladipo, Jamiu O.
Traoré, Amadou
Fassi-Fihri, Ouafaa
Ali, Abraham
Demissié, Getnet F.
Muth, Doreen
Chan, Michael C. W.
Nicholls, John M.
Meyerholz, David K.
Kuranga, Sulyman A.
Mamo, Gezahegne
Zhou, Ziqi
So, Ray T. Y.
Hemida, Maged G.
Webby, Richard J.
Roger, Francois
Rambaut, Andrew
Poon, Leo L. M.
Perlman, Stanley
Drosten, Christian
Chevalier, Veronique
Peiris, Malik
author_facet Chu, Daniel K. W.
Hui, Kenrie P. Y.
Perera, Ranawaka A. P. M.
Miguel, Eve
Niemeyer, Daniela
Zhao, Jincun
Channappanavar, Rudragouda
Dudas, Gytis
Oladipo, Jamiu O.
Traoré, Amadou
Fassi-Fihri, Ouafaa
Ali, Abraham
Demissié, Getnet F.
Muth, Doreen
Chan, Michael C. W.
Nicholls, John M.
Meyerholz, David K.
Kuranga, Sulyman A.
Mamo, Gezahegne
Zhou, Ziqi
So, Ray T. Y.
Hemida, Maged G.
Webby, Richard J.
Roger, Francois
Rambaut, Andrew
Poon, Leo L. M.
Perlman, Stanley
Drosten, Christian
Chevalier, Veronique
Peiris, Malik
author_sort Chu, Daniel K. W.
collection PubMed
description Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. Although MERS-CoV infection is ubiquitous in dromedaries across Africa as well as in the Arabian Peninsula, zoonotic disease appears confined to the Arabian Peninsula. MERS-CoVs from Africa have hitherto been poorly studied. We genetically and phenotypically characterized MERS-CoV from dromedaries sampled in Morocco, Burkina Faso, Nigeria, and Ethiopia. Viruses from Africa (clade C) are phylogenetically distinct from contemporary viruses from the Arabian Peninsula (clades A and B) but remain antigenically similar in microneutralization tests. Viruses from West (Nigeria, Burkina Faso) and North (Morocco) Africa form a subclade, C1, that shares clade-defining genetic signatures including deletions in the accessory gene ORF4b. Compared with human and camel MERS-CoV from Saudi Arabia, virus isolates from Burkina Faso (BF785) and Nigeria (Nig1657) had lower virus replication competence in Calu-3 cells and in ex vivo cultures of human bronchus and lung. BF785 replicated to lower titer in lungs of human DPP4-transduced mice. A reverse genetics-derived recombinant MERS-CoV (EMC) lacking ORF4b elicited higher type I and III IFN responses than the isogenic EMC virus in Calu-3 cells. However, ORF4b deletions may not be the major determinant of the reduced replication competence of BF785 and Nig1657. Genetic and phenotypic differences in West African viruses may be relevant to zoonotic potential. There is an urgent need for studies of MERS-CoV at the animal–human interface.
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spelling pubmed-58665762018-03-29 MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity Chu, Daniel K. W. Hui, Kenrie P. Y. Perera, Ranawaka A. P. M. Miguel, Eve Niemeyer, Daniela Zhao, Jincun Channappanavar, Rudragouda Dudas, Gytis Oladipo, Jamiu O. Traoré, Amadou Fassi-Fihri, Ouafaa Ali, Abraham Demissié, Getnet F. Muth, Doreen Chan, Michael C. W. Nicholls, John M. Meyerholz, David K. Kuranga, Sulyman A. Mamo, Gezahegne Zhou, Ziqi So, Ray T. Y. Hemida, Maged G. Webby, Richard J. Roger, Francois Rambaut, Andrew Poon, Leo L. M. Perlman, Stanley Drosten, Christian Chevalier, Veronique Peiris, Malik Proc Natl Acad Sci U S A Biological Sciences Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. Although MERS-CoV infection is ubiquitous in dromedaries across Africa as well as in the Arabian Peninsula, zoonotic disease appears confined to the Arabian Peninsula. MERS-CoVs from Africa have hitherto been poorly studied. We genetically and phenotypically characterized MERS-CoV from dromedaries sampled in Morocco, Burkina Faso, Nigeria, and Ethiopia. Viruses from Africa (clade C) are phylogenetically distinct from contemporary viruses from the Arabian Peninsula (clades A and B) but remain antigenically similar in microneutralization tests. Viruses from West (Nigeria, Burkina Faso) and North (Morocco) Africa form a subclade, C1, that shares clade-defining genetic signatures including deletions in the accessory gene ORF4b. Compared with human and camel MERS-CoV from Saudi Arabia, virus isolates from Burkina Faso (BF785) and Nigeria (Nig1657) had lower virus replication competence in Calu-3 cells and in ex vivo cultures of human bronchus and lung. BF785 replicated to lower titer in lungs of human DPP4-transduced mice. A reverse genetics-derived recombinant MERS-CoV (EMC) lacking ORF4b elicited higher type I and III IFN responses than the isogenic EMC virus in Calu-3 cells. However, ORF4b deletions may not be the major determinant of the reduced replication competence of BF785 and Nig1657. Genetic and phenotypic differences in West African viruses may be relevant to zoonotic potential. There is an urgent need for studies of MERS-CoV at the animal–human interface. National Academy of Sciences 2018-03-20 2018-03-05 /pmc/articles/PMC5866576/ /pubmed/29507189 http://dx.doi.org/10.1073/pnas.1718769115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Chu, Daniel K. W.
Hui, Kenrie P. Y.
Perera, Ranawaka A. P. M.
Miguel, Eve
Niemeyer, Daniela
Zhao, Jincun
Channappanavar, Rudragouda
Dudas, Gytis
Oladipo, Jamiu O.
Traoré, Amadou
Fassi-Fihri, Ouafaa
Ali, Abraham
Demissié, Getnet F.
Muth, Doreen
Chan, Michael C. W.
Nicholls, John M.
Meyerholz, David K.
Kuranga, Sulyman A.
Mamo, Gezahegne
Zhou, Ziqi
So, Ray T. Y.
Hemida, Maged G.
Webby, Richard J.
Roger, Francois
Rambaut, Andrew
Poon, Leo L. M.
Perlman, Stanley
Drosten, Christian
Chevalier, Veronique
Peiris, Malik
MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
title MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
title_full MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
title_fullStr MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
title_full_unstemmed MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
title_short MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
title_sort mers coronaviruses from camels in africa exhibit region-dependent genetic diversity
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866576/
https://www.ncbi.nlm.nih.gov/pubmed/29507189
http://dx.doi.org/10.1073/pnas.1718769115
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