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MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

This study purposed to explore the correlation between miR‐129‐5p and TGIF2 and their impacts on glioma cell progression. Differentially expressed miRNA was screened through microarray analysis. MiR‐129‐5p expression levels in glioma tissues and cells were measured by qRT‐PCR. CCK‐8 assay, flow cyto...

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Detalles Bibliográficos
Autores principales: Diao, Yuling, Jin, Baozhe, Huang, Liyong, Zhou, Wenke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867105/
https://www.ncbi.nlm.nih.gov/pubmed/29431269
http://dx.doi.org/10.1111/jcmm.13529
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author Diao, Yuling
Jin, Baozhe
Huang, Liyong
Zhou, Wenke
author_facet Diao, Yuling
Jin, Baozhe
Huang, Liyong
Zhou, Wenke
author_sort Diao, Yuling
collection PubMed
description This study purposed to explore the correlation between miR‐129‐5p and TGIF2 and their impacts on glioma cell progression. Differentially expressed miRNA was screened through microarray analysis. MiR‐129‐5p expression levels in glioma tissues and cells were measured by qRT‐PCR. CCK‐8 assay, flow cytometer, transwell assay and wound‐healing assay were employed to detect cell proliferation, apoptosis and cycle, invasiveness and migration, respectively. Dual‐luciferase reporting assay was performed to confirm the targeted relationship between miR‐129‐5p and TGIF2. The effects of TGIF2 expression on cell biological functions were also investigated using the indicated methods. Tumour xenograft was applied to explore the impact of miR‐129‐5p on tumorigenesis in vivo. MiR‐129‐5p expression was down‐regulated in both glioma tissues and glioma cells, while TGIF2 expression was aberrantly higher than normal level. Dual‐luciferase reporter assay validated the targeting relation between miR‐129‐5p and TGIF2. Overexpression of miR‐129‐5p or down‐regulation of TGIF2 inhibited the proliferation, invasion and migration capacity of glioma cells U87 and U251, and meanwhile blocked the cell cycle as well as induced cell apoptosis. MiR‐129‐5p overexpression repressed the tumour development in vivo. MiR‐129‐5p and TGIF2 had opposite biological functions in glioma cells. MiR‐129‐5p could inhibit glioma cell progression by targeting TGIF2, shining light for the development of target treatment for glioma.
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spelling pubmed-58671052018-04-01 MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2 Diao, Yuling Jin, Baozhe Huang, Liyong Zhou, Wenke J Cell Mol Med Original Articles This study purposed to explore the correlation between miR‐129‐5p and TGIF2 and their impacts on glioma cell progression. Differentially expressed miRNA was screened through microarray analysis. MiR‐129‐5p expression levels in glioma tissues and cells were measured by qRT‐PCR. CCK‐8 assay, flow cytometer, transwell assay and wound‐healing assay were employed to detect cell proliferation, apoptosis and cycle, invasiveness and migration, respectively. Dual‐luciferase reporting assay was performed to confirm the targeted relationship between miR‐129‐5p and TGIF2. The effects of TGIF2 expression on cell biological functions were also investigated using the indicated methods. Tumour xenograft was applied to explore the impact of miR‐129‐5p on tumorigenesis in vivo. MiR‐129‐5p expression was down‐regulated in both glioma tissues and glioma cells, while TGIF2 expression was aberrantly higher than normal level. Dual‐luciferase reporter assay validated the targeting relation between miR‐129‐5p and TGIF2. Overexpression of miR‐129‐5p or down‐regulation of TGIF2 inhibited the proliferation, invasion and migration capacity of glioma cells U87 and U251, and meanwhile blocked the cell cycle as well as induced cell apoptosis. MiR‐129‐5p overexpression repressed the tumour development in vivo. MiR‐129‐5p and TGIF2 had opposite biological functions in glioma cells. MiR‐129‐5p could inhibit glioma cell progression by targeting TGIF2, shining light for the development of target treatment for glioma. John Wiley and Sons Inc. 2018-02-12 2018-04 /pmc/articles/PMC5867105/ /pubmed/29431269 http://dx.doi.org/10.1111/jcmm.13529 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Diao, Yuling
Jin, Baozhe
Huang, Liyong
Zhou, Wenke
MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2
title MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2
title_full MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2
title_fullStr MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2
title_full_unstemmed MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2
title_short MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2
title_sort mir‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting tgif2
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867105/
https://www.ncbi.nlm.nih.gov/pubmed/29431269
http://dx.doi.org/10.1111/jcmm.13529
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