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The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools
The capacity to store urine and initiate voiding is a valued characteristic of the human urinary bladder. To maintain this feature, it is necessary that the bladder can sense when it is full and when it is time to void. The bladder has a specialized epithelium called urothelium that is believed to b...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867107/ https://www.ncbi.nlm.nih.gov/pubmed/29392898 http://dx.doi.org/10.1111/jcmm.13520 |
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author | Guan, Na N. Sharma, Nimish Hallén‐Grufman, Katarina Jager, Edwin W. H. Svennersten, Karl |
author_facet | Guan, Na N. Sharma, Nimish Hallén‐Grufman, Katarina Jager, Edwin W. H. Svennersten, Karl |
author_sort | Guan, Na N. |
collection | PubMed |
description | The capacity to store urine and initiate voiding is a valued characteristic of the human urinary bladder. To maintain this feature, it is necessary that the bladder can sense when it is full and when it is time to void. The bladder has a specialized epithelium called urothelium that is believed to be important for its sensory function. It has been suggested that autocrine ATP signalling contributes to this sensory function of the urothelium. There is well‐established evidence that ATP is released via vesicular exocytosis as well as by pannexin hemichannels upon mechanical stimulation. However, there are still many details that need elucidation and therefore there is a need for the development of new tools to further explore this fascinating field. In this work, we use new microphysiological systems to study mechanostimulation at a cellular level: a mechanostimulation microchip and a silicone‐based cell stretcher. Using these tools, we show that ATP is released upon cell stretching and that extracellular ATP contributes to a major part of Ca(2+) signalling induced by stretching in T24 cells. These results contribute to the increasing body of evidence for ATP signalling as an important component for the sensory function of urothelial cells. This encourages the development of drugs targeting P2 receptors to relieve suffering from overactive bladder disorder and incontinence. |
format | Online Article Text |
id | pubmed-5867107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58671072018-04-01 The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools Guan, Na N. Sharma, Nimish Hallén‐Grufman, Katarina Jager, Edwin W. H. Svennersten, Karl J Cell Mol Med Original Articles The capacity to store urine and initiate voiding is a valued characteristic of the human urinary bladder. To maintain this feature, it is necessary that the bladder can sense when it is full and when it is time to void. The bladder has a specialized epithelium called urothelium that is believed to be important for its sensory function. It has been suggested that autocrine ATP signalling contributes to this sensory function of the urothelium. There is well‐established evidence that ATP is released via vesicular exocytosis as well as by pannexin hemichannels upon mechanical stimulation. However, there are still many details that need elucidation and therefore there is a need for the development of new tools to further explore this fascinating field. In this work, we use new microphysiological systems to study mechanostimulation at a cellular level: a mechanostimulation microchip and a silicone‐based cell stretcher. Using these tools, we show that ATP is released upon cell stretching and that extracellular ATP contributes to a major part of Ca(2+) signalling induced by stretching in T24 cells. These results contribute to the increasing body of evidence for ATP signalling as an important component for the sensory function of urothelial cells. This encourages the development of drugs targeting P2 receptors to relieve suffering from overactive bladder disorder and incontinence. John Wiley and Sons Inc. 2018-02-01 2018-04 /pmc/articles/PMC5867107/ /pubmed/29392898 http://dx.doi.org/10.1111/jcmm.13520 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Guan, Na N. Sharma, Nimish Hallén‐Grufman, Katarina Jager, Edwin W. H. Svennersten, Karl The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools |
title | The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools |
title_full | The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools |
title_fullStr | The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools |
title_full_unstemmed | The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools |
title_short | The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools |
title_sort | role of atp signalling in response to mechanical stimulation studied in t24 cells using new microphysiological tools |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867107/ https://www.ncbi.nlm.nih.gov/pubmed/29392898 http://dx.doi.org/10.1111/jcmm.13520 |
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