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Antibiofilm Effect of DNase against Single and Mixed Species Biofilm
Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867557/ https://www.ncbi.nlm.nih.gov/pubmed/29562719 http://dx.doi.org/10.3390/foods7030042 |
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author | Sharma, Komal Pagedar Singh, Ankita |
author_facet | Sharma, Komal Pagedar Singh, Ankita |
author_sort | Sharma, Komal |
collection | PubMed |
description | Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment). The DNase I pre-treatment was optimized for P. aeruginosa PAO1 (model biofilm organism) at 10 µg/mL and post-treatment at 10 µg/mL with 15 min of contact duration. Inclusion of Mg(2+) alongside DNase I post-treatment resulted in 90% reduction in biofilm within only 5 min of contact time (irrespective of age of biofilm). On extension of these findings, DNase I was found to be less effective against mixed species biofilm than individual biofilms. DNase I can be used as potent antibiofilm agent and with further optimization can be effectively used for biofilm prevention and reduction in situ. |
format | Online Article Text |
id | pubmed-5867557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58675572018-03-27 Antibiofilm Effect of DNase against Single and Mixed Species Biofilm Sharma, Komal Pagedar Singh, Ankita Foods Article Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment). The DNase I pre-treatment was optimized for P. aeruginosa PAO1 (model biofilm organism) at 10 µg/mL and post-treatment at 10 µg/mL with 15 min of contact duration. Inclusion of Mg(2+) alongside DNase I post-treatment resulted in 90% reduction in biofilm within only 5 min of contact time (irrespective of age of biofilm). On extension of these findings, DNase I was found to be less effective against mixed species biofilm than individual biofilms. DNase I can be used as potent antibiofilm agent and with further optimization can be effectively used for biofilm prevention and reduction in situ. MDPI 2018-03-19 /pmc/articles/PMC5867557/ /pubmed/29562719 http://dx.doi.org/10.3390/foods7030042 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sharma, Komal Pagedar Singh, Ankita Antibiofilm Effect of DNase against Single and Mixed Species Biofilm |
title | Antibiofilm Effect of DNase against Single and Mixed Species Biofilm |
title_full | Antibiofilm Effect of DNase against Single and Mixed Species Biofilm |
title_fullStr | Antibiofilm Effect of DNase against Single and Mixed Species Biofilm |
title_full_unstemmed | Antibiofilm Effect of DNase against Single and Mixed Species Biofilm |
title_short | Antibiofilm Effect of DNase against Single and Mixed Species Biofilm |
title_sort | antibiofilm effect of dnase against single and mixed species biofilm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867557/ https://www.ncbi.nlm.nih.gov/pubmed/29562719 http://dx.doi.org/10.3390/foods7030042 |
work_keys_str_mv | AT sharmakomal antibiofilmeffectofdnaseagainstsingleandmixedspeciesbiofilm AT pagedarsinghankita antibiofilmeffectofdnaseagainstsingleandmixedspeciesbiofilm |