Cargando…

Antibiofilm Effect of DNase against Single and Mixed Species Biofilm

Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharma, Komal, Pagedar Singh, Ankita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867557/
https://www.ncbi.nlm.nih.gov/pubmed/29562719
http://dx.doi.org/10.3390/foods7030042
_version_ 1783308986457522176
author Sharma, Komal
Pagedar Singh, Ankita
author_facet Sharma, Komal
Pagedar Singh, Ankita
author_sort Sharma, Komal
collection PubMed
description Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment). The DNase I pre-treatment was optimized for P. aeruginosa PAO1 (model biofilm organism) at 10 µg/mL and post-treatment at 10 µg/mL with 15 min of contact duration. Inclusion of Mg(2+) alongside DNase I post-treatment resulted in 90% reduction in biofilm within only 5 min of contact time (irrespective of age of biofilm). On extension of these findings, DNase I was found to be less effective against mixed species biofilm than individual biofilms. DNase I can be used as potent antibiofilm agent and with further optimization can be effectively used for biofilm prevention and reduction in situ.
format Online
Article
Text
id pubmed-5867557
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-58675572018-03-27 Antibiofilm Effect of DNase against Single and Mixed Species Biofilm Sharma, Komal Pagedar Singh, Ankita Foods Article Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment). The DNase I pre-treatment was optimized for P. aeruginosa PAO1 (model biofilm organism) at 10 µg/mL and post-treatment at 10 µg/mL with 15 min of contact duration. Inclusion of Mg(2+) alongside DNase I post-treatment resulted in 90% reduction in biofilm within only 5 min of contact time (irrespective of age of biofilm). On extension of these findings, DNase I was found to be less effective against mixed species biofilm than individual biofilms. DNase I can be used as potent antibiofilm agent and with further optimization can be effectively used for biofilm prevention and reduction in situ. MDPI 2018-03-19 /pmc/articles/PMC5867557/ /pubmed/29562719 http://dx.doi.org/10.3390/foods7030042 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sharma, Komal
Pagedar Singh, Ankita
Antibiofilm Effect of DNase against Single and Mixed Species Biofilm
title Antibiofilm Effect of DNase against Single and Mixed Species Biofilm
title_full Antibiofilm Effect of DNase against Single and Mixed Species Biofilm
title_fullStr Antibiofilm Effect of DNase against Single and Mixed Species Biofilm
title_full_unstemmed Antibiofilm Effect of DNase against Single and Mixed Species Biofilm
title_short Antibiofilm Effect of DNase against Single and Mixed Species Biofilm
title_sort antibiofilm effect of dnase against single and mixed species biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867557/
https://www.ncbi.nlm.nih.gov/pubmed/29562719
http://dx.doi.org/10.3390/foods7030042
work_keys_str_mv AT sharmakomal antibiofilmeffectofdnaseagainstsingleandmixedspeciesbiofilm
AT pagedarsinghankita antibiofilmeffectofdnaseagainstsingleandmixedspeciesbiofilm