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Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats
After spinal cord injury (SCI), secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPCs) actively proliferate after SCI, but many of these cells undergo apoptosis. One of the factors that exacerbates secondary injury is endoplasmic reticulum (ER)...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867572/ https://www.ncbi.nlm.nih.gov/pubmed/29510579 http://dx.doi.org/10.3390/jcm7030046 |
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author | Imai, Takeshi Katoh, Hiroyuki Suyama, Kaori Kuroiwa, Masahiro Yanagisawa, Sho Watanabe, Masahiko |
author_facet | Imai, Takeshi Katoh, Hiroyuki Suyama, Kaori Kuroiwa, Masahiro Yanagisawa, Sho Watanabe, Masahiko |
author_sort | Imai, Takeshi |
collection | PubMed |
description | After spinal cord injury (SCI), secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPCs) actively proliferate after SCI, but many of these cells undergo apoptosis. One of the factors that exacerbates secondary injury is endoplasmic reticulum (ER) stress. In this study, we tested the effects of amiloride treatment on the fate of OPCs during secondary injury in rats. Amiloride is an FDA-approved diuretic for treating hypertension, which in rats enhances ER stress response and suppresses the apoptosis of glial cells after SCI. A severe contusive SCI was induced in Sprague-Dawley rats using an infinite horizon (IH)-impactor (200 kdyne). Beginning 24 h after SCI, 10 mg/kg of amiloride or phosphate buffered saline (PBS) was intraperitoneally administered daily for a period of 14 days. At 7, 14, 28, and 56 days after SCI, animals were subsequently euthanized in order to analyze the injured spinal cord. We labeled proliferating OPCs and demonstrated that amiloride treatment led to greater numbers of OPCs and oligodendrocytes in the injured spinal cord. Increased myelin basic protein (MBP) expression levels were observed, suggesting that increased numbers of mature oligodendrocytes led to improved remyelination, significantly improving motor function recovery. |
format | Online Article Text |
id | pubmed-5867572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58675722018-04-09 Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats Imai, Takeshi Katoh, Hiroyuki Suyama, Kaori Kuroiwa, Masahiro Yanagisawa, Sho Watanabe, Masahiko J Clin Med Article After spinal cord injury (SCI), secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPCs) actively proliferate after SCI, but many of these cells undergo apoptosis. One of the factors that exacerbates secondary injury is endoplasmic reticulum (ER) stress. In this study, we tested the effects of amiloride treatment on the fate of OPCs during secondary injury in rats. Amiloride is an FDA-approved diuretic for treating hypertension, which in rats enhances ER stress response and suppresses the apoptosis of glial cells after SCI. A severe contusive SCI was induced in Sprague-Dawley rats using an infinite horizon (IH)-impactor (200 kdyne). Beginning 24 h after SCI, 10 mg/kg of amiloride or phosphate buffered saline (PBS) was intraperitoneally administered daily for a period of 14 days. At 7, 14, 28, and 56 days after SCI, animals were subsequently euthanized in order to analyze the injured spinal cord. We labeled proliferating OPCs and demonstrated that amiloride treatment led to greater numbers of OPCs and oligodendrocytes in the injured spinal cord. Increased myelin basic protein (MBP) expression levels were observed, suggesting that increased numbers of mature oligodendrocytes led to improved remyelination, significantly improving motor function recovery. MDPI 2018-03-05 /pmc/articles/PMC5867572/ /pubmed/29510579 http://dx.doi.org/10.3390/jcm7030046 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Imai, Takeshi Katoh, Hiroyuki Suyama, Kaori Kuroiwa, Masahiro Yanagisawa, Sho Watanabe, Masahiko Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats |
title | Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats |
title_full | Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats |
title_fullStr | Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats |
title_full_unstemmed | Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats |
title_short | Amiloride Promotes Oligodendrocyte Survival and Remyelination after Spinal Cord Injury in Rats |
title_sort | amiloride promotes oligodendrocyte survival and remyelination after spinal cord injury in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867572/ https://www.ncbi.nlm.nih.gov/pubmed/29510579 http://dx.doi.org/10.3390/jcm7030046 |
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