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Show Yourself, Asparaginase: An Enzymatic Reaction Explained through a Hands-On Interactive Activity
[Image: see text] Determining the catalytic activity of an enzyme can be the perfect method for its identification, for example during purification procedures or for isolation purposes. Herein, we used a pharmaceutically relevant protein to bring the concept of enzymatic activity to the classroom. W...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society and Division
of Chemical Education, Inc.
2017
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867643/ https://www.ncbi.nlm.nih.gov/pubmed/29599566 http://dx.doi.org/10.1021/acs.jchemed.6b00612 |
_version_ | 1783309005976764416 |
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author | Ramirez-Paz, Josell Ortiz-Andrade, Bonny M. Griebenow, Kai Díaz-Vázquez, Liz |
author_facet | Ramirez-Paz, Josell Ortiz-Andrade, Bonny M. Griebenow, Kai Díaz-Vázquez, Liz |
author_sort | Ramirez-Paz, Josell |
collection | PubMed |
description | [Image: see text] Determining the catalytic activity of an enzyme can be the perfect method for its identification, for example during purification procedures or for isolation purposes. Herein, we used a pharmaceutically relevant protein to bring the concept of enzymatic activity to the classroom. We designed a hands-on interactive activity in which a medically relevant enzyme, asparaginase, was distinguished from a nonenzymatic protein based on its specific enzymatic activity. The experiment was carried out in the classroom, designed to impact different educational levels from elementary to high school. Our main purposes were to promote the emerging field of protein-based drugs as a source of scientific careers in bionanotechnology and to show the students an image of a “scientist” as that of a common and educated person working in an exciting profession. In addition of being inexpensive, this activity proved to be adaptable for various educational levels and can be easily implemented in different scenarios, for example, scientific fairs, some schools, and so forth. |
format | Online Article Text |
id | pubmed-5867643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical
Society and Division
of Chemical Education, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58676432018-03-27 Show Yourself, Asparaginase: An Enzymatic Reaction Explained through a Hands-On Interactive Activity Ramirez-Paz, Josell Ortiz-Andrade, Bonny M. Griebenow, Kai Díaz-Vázquez, Liz J Chem Educ [Image: see text] Determining the catalytic activity of an enzyme can be the perfect method for its identification, for example during purification procedures or for isolation purposes. Herein, we used a pharmaceutically relevant protein to bring the concept of enzymatic activity to the classroom. We designed a hands-on interactive activity in which a medically relevant enzyme, asparaginase, was distinguished from a nonenzymatic protein based on its specific enzymatic activity. The experiment was carried out in the classroom, designed to impact different educational levels from elementary to high school. Our main purposes were to promote the emerging field of protein-based drugs as a source of scientific careers in bionanotechnology and to show the students an image of a “scientist” as that of a common and educated person working in an exciting profession. In addition of being inexpensive, this activity proved to be adaptable for various educational levels and can be easily implemented in different scenarios, for example, scientific fairs, some schools, and so forth. American Chemical Society and Division of Chemical Education, Inc. 2017-03-22 2017-06-13 /pmc/articles/PMC5867643/ /pubmed/29599566 http://dx.doi.org/10.1021/acs.jchemed.6b00612 Text en Copyright © 2017 American Chemical Society and Division of Chemical Education, Inc. This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Ramirez-Paz, Josell Ortiz-Andrade, Bonny M. Griebenow, Kai Díaz-Vázquez, Liz Show Yourself, Asparaginase: An Enzymatic Reaction Explained through a Hands-On Interactive Activity |
title | Show Yourself, Asparaginase: An Enzymatic Reaction
Explained through a Hands-On Interactive Activity |
title_full | Show Yourself, Asparaginase: An Enzymatic Reaction
Explained through a Hands-On Interactive Activity |
title_fullStr | Show Yourself, Asparaginase: An Enzymatic Reaction
Explained through a Hands-On Interactive Activity |
title_full_unstemmed | Show Yourself, Asparaginase: An Enzymatic Reaction
Explained through a Hands-On Interactive Activity |
title_short | Show Yourself, Asparaginase: An Enzymatic Reaction
Explained through a Hands-On Interactive Activity |
title_sort | show yourself, asparaginase: an enzymatic reaction
explained through a hands-on interactive activity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867643/ https://www.ncbi.nlm.nih.gov/pubmed/29599566 http://dx.doi.org/10.1021/acs.jchemed.6b00612 |
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