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Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer

The velvet family proteins have been shown to play critical roles in fungal secondary metabolism and development. However, variations of the roles have been observed in different fungi. We report here the observation on the role of three velvet complex components VeA, VelB, and LaeA in Pestalotiopsi...

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Autores principales: Akhberdi, Oren, Zhang, Qian, Wang, Dan, Wang, Haichuan, Hao, Xiaoran, Liu, Yanjie, Wei, Dongsheng, Zhu, Xudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867885/
https://www.ncbi.nlm.nih.gov/pubmed/29538316
http://dx.doi.org/10.3390/genes9030164
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author Akhberdi, Oren
Zhang, Qian
Wang, Dan
Wang, Haichuan
Hao, Xiaoran
Liu, Yanjie
Wei, Dongsheng
Zhu, Xudong
author_facet Akhberdi, Oren
Zhang, Qian
Wang, Dan
Wang, Haichuan
Hao, Xiaoran
Liu, Yanjie
Wei, Dongsheng
Zhu, Xudong
author_sort Akhberdi, Oren
collection PubMed
description The velvet family proteins have been shown to play critical roles in fungal secondary metabolism and development. However, variations of the roles have been observed in different fungi. We report here the observation on the role of three velvet complex components VeA, VelB, and LaeA in Pestalotiopsis microspora, a formerly reported taxol-producing fungus. Deletion of individual members led to the retardation of vegetative growth and sporulation and pigmentation, suggesting critical roles in these processes. The mutant strain △velB appeared hypersensitive to osmotic stress and the dye Congo red, whereas △veA and △laeA were little affected by the pressures, suggesting only velB was required for the integrity of the cell wall. Importantly, we found that the genes played distinct roles in the biosynthesis of secondary metabolites in P. microspora. For instance, the production of pestalotiollide B, a previously characterized polyketide, required velB and laeA. In contrast, the veA gene appeared to inhibit the pestalotiollide B (PB) role in its biosynthesis. This study suggests that the three components of the velvet complex are important global regulators, but with distinct roles in hyphal growth, asexual production, and secondary metabolism in P. microspora. This work provides information for further understanding the biosynthesis of secondary metabolism in the fungus.
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spelling pubmed-58678852018-03-27 Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer Akhberdi, Oren Zhang, Qian Wang, Dan Wang, Haichuan Hao, Xiaoran Liu, Yanjie Wei, Dongsheng Zhu, Xudong Genes (Basel) Article The velvet family proteins have been shown to play critical roles in fungal secondary metabolism and development. However, variations of the roles have been observed in different fungi. We report here the observation on the role of three velvet complex components VeA, VelB, and LaeA in Pestalotiopsis microspora, a formerly reported taxol-producing fungus. Deletion of individual members led to the retardation of vegetative growth and sporulation and pigmentation, suggesting critical roles in these processes. The mutant strain △velB appeared hypersensitive to osmotic stress and the dye Congo red, whereas △veA and △laeA were little affected by the pressures, suggesting only velB was required for the integrity of the cell wall. Importantly, we found that the genes played distinct roles in the biosynthesis of secondary metabolites in P. microspora. For instance, the production of pestalotiollide B, a previously characterized polyketide, required velB and laeA. In contrast, the veA gene appeared to inhibit the pestalotiollide B (PB) role in its biosynthesis. This study suggests that the three components of the velvet complex are important global regulators, but with distinct roles in hyphal growth, asexual production, and secondary metabolism in P. microspora. This work provides information for further understanding the biosynthesis of secondary metabolism in the fungus. MDPI 2018-03-14 /pmc/articles/PMC5867885/ /pubmed/29538316 http://dx.doi.org/10.3390/genes9030164 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akhberdi, Oren
Zhang, Qian
Wang, Dan
Wang, Haichuan
Hao, Xiaoran
Liu, Yanjie
Wei, Dongsheng
Zhu, Xudong
Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer
title Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer
title_full Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer
title_fullStr Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer
title_full_unstemmed Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer
title_short Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer
title_sort distinct roles of velvet complex in the development, stress tolerance, and secondary metabolism in pestalotiopsis microspora, a taxol producer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5867885/
https://www.ncbi.nlm.nih.gov/pubmed/29538316
http://dx.doi.org/10.3390/genes9030164
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