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Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice
INTRODUCTION: Nonalcoholic fatty liver disease (NAFLD) is a condition in which excess fat accumulates in the liver of a patient without a history of alcohol abuse. Fatty liver fibrosis, a severe form of NAFLD, is a key step which can be reversed by effective medical intervention. This paper aims to...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868672/ https://www.ncbi.nlm.nih.gov/pubmed/29593815 http://dx.doi.org/10.5114/aoms.2016.63260 |
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author | Yuan, Ding Xiang, Tingting Huo, Yuanxiu Liu, Chaoqi Wang, Ting Zhou, Zhiyong Dun, Yaoyan Zhao, Haixia Zhang, Changcheng |
author_facet | Yuan, Ding Xiang, Tingting Huo, Yuanxiu Liu, Chaoqi Wang, Ting Zhou, Zhiyong Dun, Yaoyan Zhao, Haixia Zhang, Changcheng |
author_sort | Yuan, Ding |
collection | PubMed |
description | INTRODUCTION: Nonalcoholic fatty liver disease (NAFLD) is a condition in which excess fat accumulates in the liver of a patient without a history of alcohol abuse. Fatty liver fibrosis, a severe form of NAFLD, is a key step which can be reversed by effective medical intervention. This paper aims to describe the protective role and mechanisms of action of total saponins of Panax japonicus (SPJ) against fatty liver fibrosis in mice. In this study, fatty liver fibrosis was induced by a high-fat (HF) diet combined with intraperitoneal injection of porcine serum. MATERIAL AND METHODS: The fatty liver fibrosis model was induced by HF diet combined with intraperitoneal injection of porcine serum. The endoplasmic reticulum stress (ERS) response and C/EBP homologous protein (CHOP) and p-Jun N-terminal kinase (JNK)-mediated apoptosis and inflammation were assessed by serum biochemistry, hematoxylin-eosin (H + E), Masson and electronic microscopy staining, Hyp content detection, Western blotting and real time polymerase chain reaction (RT-PCR). RESULTS: Saponins of Panax japonicus could significantly improve liver function and decrease the lipid level in the serum. The liver steatosis, collagen fibers and inflammatory cell infiltration were significantly improved in the SPJ group according to microscope observation. The RT-PCR analysis revealed that the collagen I (Coll), α smooth muscle actin (α-SMA), tissue inhibitors of MMPs (TIMP), CHOP and GRP78 mRNA expression levels were distinctly weakened by SPJ treatment; and western blotting analysis indicated that the phosphorylated JNK (p-JNK), Coll and 78 kD glucose-regulated protein (GRP78) protein expression levels were significantly alleviated, which might be associated with the inhibition of the ERS response and the CHOP and JNK-mediated apoptosis and inflammation pathway. CONCLUSIONS: Based on this research, SPJ as a preventive medicine has great potential in prevention of liver fibrosis. |
format | Online Article Text |
id | pubmed-5868672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-58686722018-03-28 Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice Yuan, Ding Xiang, Tingting Huo, Yuanxiu Liu, Chaoqi Wang, Ting Zhou, Zhiyong Dun, Yaoyan Zhao, Haixia Zhang, Changcheng Arch Med Sci Experimental Research INTRODUCTION: Nonalcoholic fatty liver disease (NAFLD) is a condition in which excess fat accumulates in the liver of a patient without a history of alcohol abuse. Fatty liver fibrosis, a severe form of NAFLD, is a key step which can be reversed by effective medical intervention. This paper aims to describe the protective role and mechanisms of action of total saponins of Panax japonicus (SPJ) against fatty liver fibrosis in mice. In this study, fatty liver fibrosis was induced by a high-fat (HF) diet combined with intraperitoneal injection of porcine serum. MATERIAL AND METHODS: The fatty liver fibrosis model was induced by HF diet combined with intraperitoneal injection of porcine serum. The endoplasmic reticulum stress (ERS) response and C/EBP homologous protein (CHOP) and p-Jun N-terminal kinase (JNK)-mediated apoptosis and inflammation were assessed by serum biochemistry, hematoxylin-eosin (H + E), Masson and electronic microscopy staining, Hyp content detection, Western blotting and real time polymerase chain reaction (RT-PCR). RESULTS: Saponins of Panax japonicus could significantly improve liver function and decrease the lipid level in the serum. The liver steatosis, collagen fibers and inflammatory cell infiltration were significantly improved in the SPJ group according to microscope observation. The RT-PCR analysis revealed that the collagen I (Coll), α smooth muscle actin (α-SMA), tissue inhibitors of MMPs (TIMP), CHOP and GRP78 mRNA expression levels were distinctly weakened by SPJ treatment; and western blotting analysis indicated that the phosphorylated JNK (p-JNK), Coll and 78 kD glucose-regulated protein (GRP78) protein expression levels were significantly alleviated, which might be associated with the inhibition of the ERS response and the CHOP and JNK-mediated apoptosis and inflammation pathway. CONCLUSIONS: Based on this research, SPJ as a preventive medicine has great potential in prevention of liver fibrosis. Termedia Publishing House 2016-10-26 2018-03 /pmc/articles/PMC5868672/ /pubmed/29593815 http://dx.doi.org/10.5114/aoms.2016.63260 Text en Copyright: © 2016 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Experimental Research Yuan, Ding Xiang, Tingting Huo, Yuanxiu Liu, Chaoqi Wang, Ting Zhou, Zhiyong Dun, Yaoyan Zhao, Haixia Zhang, Changcheng Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice |
title | Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice |
title_full | Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice |
title_fullStr | Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice |
title_full_unstemmed | Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice |
title_short | Preventive effects of total saponins of Panax japonicus on fatty liver fibrosis in mice |
title_sort | preventive effects of total saponins of panax japonicus on fatty liver fibrosis in mice |
topic | Experimental Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868672/ https://www.ncbi.nlm.nih.gov/pubmed/29593815 http://dx.doi.org/10.5114/aoms.2016.63260 |
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