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Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy
INTRODUCTION: Morphea is a chronic autoimmune disease characterized by fibrosis of the skin. Dendritic cells (DC) and regulatory T cells (Tregs) play a significant role in development of autoimmune and tolerance mechanisms. The aim of the study was to establish the expression of selected genes of pl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868677/ https://www.ncbi.nlm.nih.gov/pubmed/29593811 http://dx.doi.org/10.5114/aoms.2018.73469 |
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author | Osmola-Mańkowska, Agnieszka J. Teresiak-Mikołajczak, Ewa Kowalczyk, Michał J. Żaba, Ryszard W. Adamski, Zygmunt Dańczak-Pazdrowska, Aleksandra |
author_facet | Osmola-Mańkowska, Agnieszka J. Teresiak-Mikołajczak, Ewa Kowalczyk, Michał J. Żaba, Ryszard W. Adamski, Zygmunt Dańczak-Pazdrowska, Aleksandra |
author_sort | Osmola-Mańkowska, Agnieszka J. |
collection | PubMed |
description | INTRODUCTION: Morphea is a chronic autoimmune disease characterized by fibrosis of the skin. Dendritic cells (DC) and regulatory T cells (Tregs) play a significant role in development of autoimmune and tolerance mechanisms. The aim of the study was to establish the expression of selected genes of plasmacytoid and myeloid DC, Treg cells, and the microenvironment of cytokines (interleukin-17A (IL-17A), transforming growth factor β (TGF-β)) in blood and skin of morphea patients. In addition, the effect of UVA1 phototherapy on expression of the aforementioned genes was evaluated. MATERIAL AND METHODS: The study was performed on 15 blood and 10 skin samples from patients with morphea. The evaluation included expression of CLEC4C (C-type lectin domain family 4, member C receptor), Lymphocyte antigen 75 (LY75), Forkhead box p3 (foxp3) transcription factor, IL-17A and TGF-β genes in peripheral blood mononuclear cells (PBMC) and in skin samples both before and after UVA1 phototherapy using real-time polymerase chain reaction. RESULTS: The study revealed lower expression of CLEC4C before (p = 0.010) and after (p = 0.009) phototherapy and lower expression of IL-17A before (p = 0.038) phototherapy in PBMC of patients with morphea vs. the control group. Expression of CLEC4C in PBMC correlated negatively (rho = –0.90; p = 0.001) with activity of disease after phototherapy. No significant differences were found between expression of analysed genes before and after UVA1 therapy in PBMC and skin of morphea patients. CONCLUSIONS: The results do not confirm the involvement of analysed subsets of DC and Tregs in UVA1 phototherapy in morphea, but point to CLEC4C as a possible biomarker associated with the disease activity. |
format | Online Article Text |
id | pubmed-5868677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-58686772018-03-28 Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy Osmola-Mańkowska, Agnieszka J. Teresiak-Mikołajczak, Ewa Kowalczyk, Michał J. Żaba, Ryszard W. Adamski, Zygmunt Dańczak-Pazdrowska, Aleksandra Arch Med Sci Basic Research INTRODUCTION: Morphea is a chronic autoimmune disease characterized by fibrosis of the skin. Dendritic cells (DC) and regulatory T cells (Tregs) play a significant role in development of autoimmune and tolerance mechanisms. The aim of the study was to establish the expression of selected genes of plasmacytoid and myeloid DC, Treg cells, and the microenvironment of cytokines (interleukin-17A (IL-17A), transforming growth factor β (TGF-β)) in blood and skin of morphea patients. In addition, the effect of UVA1 phototherapy on expression of the aforementioned genes was evaluated. MATERIAL AND METHODS: The study was performed on 15 blood and 10 skin samples from patients with morphea. The evaluation included expression of CLEC4C (C-type lectin domain family 4, member C receptor), Lymphocyte antigen 75 (LY75), Forkhead box p3 (foxp3) transcription factor, IL-17A and TGF-β genes in peripheral blood mononuclear cells (PBMC) and in skin samples both before and after UVA1 phototherapy using real-time polymerase chain reaction. RESULTS: The study revealed lower expression of CLEC4C before (p = 0.010) and after (p = 0.009) phototherapy and lower expression of IL-17A before (p = 0.038) phototherapy in PBMC of patients with morphea vs. the control group. Expression of CLEC4C in PBMC correlated negatively (rho = –0.90; p = 0.001) with activity of disease after phototherapy. No significant differences were found between expression of analysed genes before and after UVA1 therapy in PBMC and skin of morphea patients. CONCLUSIONS: The results do not confirm the involvement of analysed subsets of DC and Tregs in UVA1 phototherapy in morphea, but point to CLEC4C as a possible biomarker associated with the disease activity. Termedia Publishing House 2018-02-21 2018-03 /pmc/articles/PMC5868677/ /pubmed/29593811 http://dx.doi.org/10.5114/aoms.2018.73469 Text en Copyright: © 2018 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Basic Research Osmola-Mańkowska, Agnieszka J. Teresiak-Mikołajczak, Ewa Kowalczyk, Michał J. Żaba, Ryszard W. Adamski, Zygmunt Dańczak-Pazdrowska, Aleksandra Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy |
title | Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy |
title_full | Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy |
title_fullStr | Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy |
title_full_unstemmed | Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy |
title_short | Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy |
title_sort | expression of selected genes of dendritic and treg cells in blood and skin of morphea patients treated with uva1 phototherapy |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868677/ https://www.ncbi.nlm.nih.gov/pubmed/29593811 http://dx.doi.org/10.5114/aoms.2018.73469 |
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