Cargando…

Compact in-line lensfree digital holographic microscope

Phase imaging provides intensity contrast to visualize transparent samples such as found in biology without any staining. Among them, digital holographic microscopy (DHM) is a well-known quantitative phase method. Lensfree implementations of DHMs offer the added advantage to provide large field of v...

Descripción completa

Detalles Bibliográficos
Autores principales: Rostykus, Manon, Soulez, Ferréol, Unser, Michael, Moser, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869056/
https://www.ncbi.nlm.nih.gov/pubmed/29162547
http://dx.doi.org/10.1016/j.ymeth.2017.11.008
_version_ 1783309229636976640
author Rostykus, Manon
Soulez, Ferréol
Unser, Michael
Moser, Christophe
author_facet Rostykus, Manon
Soulez, Ferréol
Unser, Michael
Moser, Christophe
author_sort Rostykus, Manon
collection PubMed
description Phase imaging provides intensity contrast to visualize transparent samples such as found in biology without any staining. Among them, digital holographic microscopy (DHM) is a well-known quantitative phase method. Lensfree implementations of DHMs offer the added advantage to provide large field of views (several mm(2) compared to several hundred μm(2)) and more compact setups that traditional DHM which have high quality microscope objectives. In this article, a lensfree DHM is presented using a side illumination technique in order to further reduce the device size. Its practical use is described and results on a transparent (phase only) sample are shown.
format Online
Article
Text
id pubmed-5869056
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Academic Press
record_format MEDLINE/PubMed
spelling pubmed-58690562018-03-27 Compact in-line lensfree digital holographic microscope Rostykus, Manon Soulez, Ferréol Unser, Michael Moser, Christophe Methods Article Phase imaging provides intensity contrast to visualize transparent samples such as found in biology without any staining. Among them, digital holographic microscopy (DHM) is a well-known quantitative phase method. Lensfree implementations of DHMs offer the added advantage to provide large field of views (several mm(2) compared to several hundred μm(2)) and more compact setups that traditional DHM which have high quality microscope objectives. In this article, a lensfree DHM is presented using a side illumination technique in order to further reduce the device size. Its practical use is described and results on a transparent (phase only) sample are shown. Academic Press 2018-03-01 /pmc/articles/PMC5869056/ /pubmed/29162547 http://dx.doi.org/10.1016/j.ymeth.2017.11.008 Text en © 2017 The Authors. Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Rostykus, Manon
Soulez, Ferréol
Unser, Michael
Moser, Christophe
Compact in-line lensfree digital holographic microscope
title Compact in-line lensfree digital holographic microscope
title_full Compact in-line lensfree digital holographic microscope
title_fullStr Compact in-line lensfree digital holographic microscope
title_full_unstemmed Compact in-line lensfree digital holographic microscope
title_short Compact in-line lensfree digital holographic microscope
title_sort compact in-line lensfree digital holographic microscope
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869056/
https://www.ncbi.nlm.nih.gov/pubmed/29162547
http://dx.doi.org/10.1016/j.ymeth.2017.11.008
work_keys_str_mv AT rostykusmanon compactinlinelensfreedigitalholographicmicroscope
AT soulezferreol compactinlinelensfreedigitalholographicmicroscope
AT unsermichael compactinlinelensfreedigitalholographicmicroscope
AT moserchristophe compactinlinelensfreedigitalholographicmicroscope