Cargando…
Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen wa...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869499/ https://www.ncbi.nlm.nih.gov/pubmed/29494530 http://dx.doi.org/10.3390/v10030106 |
_version_ | 1783309296055877632 |
---|---|
author | Hou, Xingyu Jiang, Xinpeng Jiang, Yanping Tang, Lijie Xu, Yigang Qiao, Xinyuan Liu, Min Cui, Wen Ma, Guangpeng Li, Yijing |
author_facet | Hou, Xingyu Jiang, Xinpeng Jiang, Yanping Tang, Lijie Xu, Yigang Qiao, Xinyuan Liu, Min Cui, Wen Ma, Guangpeng Li, Yijing |
author_sort | Hou, Xingyu |
collection | PubMed |
description | Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen was developed. This vaccine induced significant levels of anti-PEDV specific IgG and IgA antibody responses in mice, indicating a potential strategy against PEDV infection. In this study, pPG-COE-DCpep/L393 was used for oral vaccination of newborn piglets against PEDV. We then assessed the immune responses and protection efficacy of pPG-COE-DCpep/L393. An indirect enzyme-linked immunosorbent assay (ELISA) showed that the recombinant Lactobacillus vaccine elicits a specific systemic and mucosal immune response. The T-helper cells mediated by pPG-COE-DCpep/L393 and PEDV infection display a Th1 phenotype. The histopathological results showed that pPG-COE-DCpep/L393 promotes lymphocyte proliferation and effectively protects piglets against PEDV infection. The transforming growth factor-β level indicated that the recombinant Lactobacillus vaccine plays a role in anti-inflammatory responses in mesenteric lymph nodes during PEDV infection. These results show that pPG-COE-DCpep/L393 is a potential vaccine against PEDV infection. |
format | Online Article Text |
id | pubmed-5869499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58694992018-03-28 Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets Hou, Xingyu Jiang, Xinpeng Jiang, Yanping Tang, Lijie Xu, Yigang Qiao, Xinyuan Liu, Min Cui, Wen Ma, Guangpeng Li, Yijing Viruses Article Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen was developed. This vaccine induced significant levels of anti-PEDV specific IgG and IgA antibody responses in mice, indicating a potential strategy against PEDV infection. In this study, pPG-COE-DCpep/L393 was used for oral vaccination of newborn piglets against PEDV. We then assessed the immune responses and protection efficacy of pPG-COE-DCpep/L393. An indirect enzyme-linked immunosorbent assay (ELISA) showed that the recombinant Lactobacillus vaccine elicits a specific systemic and mucosal immune response. The T-helper cells mediated by pPG-COE-DCpep/L393 and PEDV infection display a Th1 phenotype. The histopathological results showed that pPG-COE-DCpep/L393 promotes lymphocyte proliferation and effectively protects piglets against PEDV infection. The transforming growth factor-β level indicated that the recombinant Lactobacillus vaccine plays a role in anti-inflammatory responses in mesenteric lymph nodes during PEDV infection. These results show that pPG-COE-DCpep/L393 is a potential vaccine against PEDV infection. MDPI 2018-03-01 /pmc/articles/PMC5869499/ /pubmed/29494530 http://dx.doi.org/10.3390/v10030106 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hou, Xingyu Jiang, Xinpeng Jiang, Yanping Tang, Lijie Xu, Yigang Qiao, Xinyuan Liu, Min Cui, Wen Ma, Guangpeng Li, Yijing Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets |
title | Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets |
title_full | Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets |
title_fullStr | Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets |
title_full_unstemmed | Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets |
title_short | Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets |
title_sort | oral immunization against pedv with recombinant lactobacillus casei expressing dendritic cell-targeting peptide fusing coe protein of pedv in piglets |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869499/ https://www.ncbi.nlm.nih.gov/pubmed/29494530 http://dx.doi.org/10.3390/v10030106 |
work_keys_str_mv | AT houxingyu oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT jiangxinpeng oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT jiangyanping oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT tanglijie oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT xuyigang oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT qiaoxinyuan oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT liumin oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT cuiwen oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT maguangpeng oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets AT liyijing oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets |