Cargando…

Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets

Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen wa...

Descripción completa

Detalles Bibliográficos
Autores principales: Hou, Xingyu, Jiang, Xinpeng, Jiang, Yanping, Tang, Lijie, Xu, Yigang, Qiao, Xinyuan, Liu, Min, Cui, Wen, Ma, Guangpeng, Li, Yijing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869499/
https://www.ncbi.nlm.nih.gov/pubmed/29494530
http://dx.doi.org/10.3390/v10030106
_version_ 1783309296055877632
author Hou, Xingyu
Jiang, Xinpeng
Jiang, Yanping
Tang, Lijie
Xu, Yigang
Qiao, Xinyuan
Liu, Min
Cui, Wen
Ma, Guangpeng
Li, Yijing
author_facet Hou, Xingyu
Jiang, Xinpeng
Jiang, Yanping
Tang, Lijie
Xu, Yigang
Qiao, Xinyuan
Liu, Min
Cui, Wen
Ma, Guangpeng
Li, Yijing
author_sort Hou, Xingyu
collection PubMed
description Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen was developed. This vaccine induced significant levels of anti-PEDV specific IgG and IgA antibody responses in mice, indicating a potential strategy against PEDV infection. In this study, pPG-COE-DCpep/L393 was used for oral vaccination of newborn piglets against PEDV. We then assessed the immune responses and protection efficacy of pPG-COE-DCpep/L393. An indirect enzyme-linked immunosorbent assay (ELISA) showed that the recombinant Lactobacillus vaccine elicits a specific systemic and mucosal immune response. The T-helper cells mediated by pPG-COE-DCpep/L393 and PEDV infection display a Th1 phenotype. The histopathological results showed that pPG-COE-DCpep/L393 promotes lymphocyte proliferation and effectively protects piglets against PEDV infection. The transforming growth factor-β level indicated that the recombinant Lactobacillus vaccine plays a role in anti-inflammatory responses in mesenteric lymph nodes during PEDV infection. These results show that pPG-COE-DCpep/L393 is a potential vaccine against PEDV infection.
format Online
Article
Text
id pubmed-5869499
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-58694992018-03-28 Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets Hou, Xingyu Jiang, Xinpeng Jiang, Yanping Tang, Lijie Xu, Yigang Qiao, Xinyuan Liu, Min Cui, Wen Ma, Guangpeng Li, Yijing Viruses Article Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen was developed. This vaccine induced significant levels of anti-PEDV specific IgG and IgA antibody responses in mice, indicating a potential strategy against PEDV infection. In this study, pPG-COE-DCpep/L393 was used for oral vaccination of newborn piglets against PEDV. We then assessed the immune responses and protection efficacy of pPG-COE-DCpep/L393. An indirect enzyme-linked immunosorbent assay (ELISA) showed that the recombinant Lactobacillus vaccine elicits a specific systemic and mucosal immune response. The T-helper cells mediated by pPG-COE-DCpep/L393 and PEDV infection display a Th1 phenotype. The histopathological results showed that pPG-COE-DCpep/L393 promotes lymphocyte proliferation and effectively protects piglets against PEDV infection. The transforming growth factor-β level indicated that the recombinant Lactobacillus vaccine plays a role in anti-inflammatory responses in mesenteric lymph nodes during PEDV infection. These results show that pPG-COE-DCpep/L393 is a potential vaccine against PEDV infection. MDPI 2018-03-01 /pmc/articles/PMC5869499/ /pubmed/29494530 http://dx.doi.org/10.3390/v10030106 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hou, Xingyu
Jiang, Xinpeng
Jiang, Yanping
Tang, Lijie
Xu, Yigang
Qiao, Xinyuan
Liu, Min
Cui, Wen
Ma, Guangpeng
Li, Yijing
Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
title Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
title_full Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
title_fullStr Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
title_full_unstemmed Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
title_short Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
title_sort oral immunization against pedv with recombinant lactobacillus casei expressing dendritic cell-targeting peptide fusing coe protein of pedv in piglets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869499/
https://www.ncbi.nlm.nih.gov/pubmed/29494530
http://dx.doi.org/10.3390/v10030106
work_keys_str_mv AT houxingyu oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT jiangxinpeng oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT jiangyanping oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT tanglijie oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT xuyigang oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT qiaoxinyuan oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT liumin oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT cuiwen oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT maguangpeng oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets
AT liyijing oralimmunizationagainstpedvwithrecombinantlactobacilluscaseiexpressingdendriticcelltargetingpeptidefusingcoeproteinofpedvinpiglets