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Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland

Circoviruses are circular single-stranded DNA (ssDNA) viruses that infect a variety of animals, both domestic and wild. Circovirus infection in birds is associated with immunosuppression and this in turn predisposes the infected animals to secondary infections that can lead to mortality. Farmed gees...

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Autores principales: Stenzel, Tomasz, Dziewulska, Daria, Muhire, Brejnev M., Hartnady, Penelope, Kraberger, Simona, Martin, Darren P., Varsani, Arvind
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869500/
https://www.ncbi.nlm.nih.gov/pubmed/29498637
http://dx.doi.org/10.3390/v10030107
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author Stenzel, Tomasz
Dziewulska, Daria
Muhire, Brejnev M.
Hartnady, Penelope
Kraberger, Simona
Martin, Darren P.
Varsani, Arvind
author_facet Stenzel, Tomasz
Dziewulska, Daria
Muhire, Brejnev M.
Hartnady, Penelope
Kraberger, Simona
Martin, Darren P.
Varsani, Arvind
author_sort Stenzel, Tomasz
collection PubMed
description Circoviruses are circular single-stranded DNA (ssDNA) viruses that infect a variety of animals, both domestic and wild. Circovirus infection in birds is associated with immunosuppression and this in turn predisposes the infected animals to secondary infections that can lead to mortality. Farmed geese (Anser anser) in many parts of the world are infected with circoviruses. The majority of the current genomic information for goose circoviruses (GoCVs) (n = 40) are from birds sampled in China and Taiwan, and only two genome sequences are available from Europe (Germany and Poland). In this study, we sampled 23 wild and 19 domestic geese from the Gopło Lake area in Poland. We determined the genomes of GoCV from 21 geese; 14 domestic Greylag geese (Anser anser), three wild Greylag geese (A. anser), three bean geese (A. fabalis), and one white fronted goose (A. albifrons). These genomes share 83–95% nucleotide pairwise identities with previously identified GoCV genomes, most are recombinants with exchanged fragment sizes up to 50% of the genome. Higher diversity levels can be seen within the genomes from domestic geese compared with those from wild geese. In the GoCV capsid protein (cp) and replication associated protein (rep) gene sequences we found that episodic positive selection appears to largely mirror those of beak and feather disease virus and pigeon circovirus. Analysis of the secondary structure of the ssDNA genome revealed a conserved stem-loop structure with the G-C rich stem having a high degree of negative selection on these nucleotides.
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spelling pubmed-58695002018-03-28 Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland Stenzel, Tomasz Dziewulska, Daria Muhire, Brejnev M. Hartnady, Penelope Kraberger, Simona Martin, Darren P. Varsani, Arvind Viruses Article Circoviruses are circular single-stranded DNA (ssDNA) viruses that infect a variety of animals, both domestic and wild. Circovirus infection in birds is associated with immunosuppression and this in turn predisposes the infected animals to secondary infections that can lead to mortality. Farmed geese (Anser anser) in many parts of the world are infected with circoviruses. The majority of the current genomic information for goose circoviruses (GoCVs) (n = 40) are from birds sampled in China and Taiwan, and only two genome sequences are available from Europe (Germany and Poland). In this study, we sampled 23 wild and 19 domestic geese from the Gopło Lake area in Poland. We determined the genomes of GoCV from 21 geese; 14 domestic Greylag geese (Anser anser), three wild Greylag geese (A. anser), three bean geese (A. fabalis), and one white fronted goose (A. albifrons). These genomes share 83–95% nucleotide pairwise identities with previously identified GoCV genomes, most are recombinants with exchanged fragment sizes up to 50% of the genome. Higher diversity levels can be seen within the genomes from domestic geese compared with those from wild geese. In the GoCV capsid protein (cp) and replication associated protein (rep) gene sequences we found that episodic positive selection appears to largely mirror those of beak and feather disease virus and pigeon circovirus. Analysis of the secondary structure of the ssDNA genome revealed a conserved stem-loop structure with the G-C rich stem having a high degree of negative selection on these nucleotides. MDPI 2018-03-02 /pmc/articles/PMC5869500/ /pubmed/29498637 http://dx.doi.org/10.3390/v10030107 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stenzel, Tomasz
Dziewulska, Daria
Muhire, Brejnev M.
Hartnady, Penelope
Kraberger, Simona
Martin, Darren P.
Varsani, Arvind
Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland
title Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland
title_full Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland
title_fullStr Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland
title_full_unstemmed Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland
title_short Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland
title_sort recombinant goose circoviruses circulating in domesticated and wild geese in poland
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869500/
https://www.ncbi.nlm.nih.gov/pubmed/29498637
http://dx.doi.org/10.3390/v10030107
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