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Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering

For biomedical applications such as soft tissue engineering, plant proteins are becoming increasingly attractive. Zein, a class of prolamine proteins found in corn, offers excellent properties for application in the human body, but has inferior mechanical properties and lacks aqueous stability. In t...

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Autores principales: Vogt, Lena, Liverani, Liliana, Roether, Judith A., Boccaccini, Aldo R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869641/
https://www.ncbi.nlm.nih.gov/pubmed/29518041
http://dx.doi.org/10.3390/nano8030150
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author Vogt, Lena
Liverani, Liliana
Roether, Judith A.
Boccaccini, Aldo R.
author_facet Vogt, Lena
Liverani, Liliana
Roether, Judith A.
Boccaccini, Aldo R.
author_sort Vogt, Lena
collection PubMed
description For biomedical applications such as soft tissue engineering, plant proteins are becoming increasingly attractive. Zein, a class of prolamine proteins found in corn, offers excellent properties for application in the human body, but has inferior mechanical properties and lacks aqueous stability. In this study, electrospun scaffolds from neat zein and zein blended with prepolymer and mildly cross-linked poly(glycerol sebacate) (PGS) were fabricated. Less toxic solvents like acetic acid and ethanol were used. The morphological, physiochemical and degradation properties of the as-spun fiber mats were determined. Neat zein and zein-PGS fiber mats with high zein concentration (24 wt % and 27 wt %) showed defect-free microstructures. The average fiber diameter decreased with increasing PGS amount from 0.7 ± 0.2 µm to 0.09 ± 0.03 µm. The addition of PGS to zein resulted in a seven-fold increase in ultimate tensile strength and a four-fold increase in failure strain, whereas the Young’s Modulus did not change significantly. Degradation tests in phosphate buffered saline revealed the morphological instability of zein containing fiber mats in contact with aqueous media. Therefore, the fibers were in situ cross-linked with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC)/N-Hydroxysuccinimide (NHS), which led to improved morphological stability in aqueous environment. The novel fibers have suitable properties for application in soft tissue engineering.
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spelling pubmed-58696412018-03-28 Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering Vogt, Lena Liverani, Liliana Roether, Judith A. Boccaccini, Aldo R. Nanomaterials (Basel) Article For biomedical applications such as soft tissue engineering, plant proteins are becoming increasingly attractive. Zein, a class of prolamine proteins found in corn, offers excellent properties for application in the human body, but has inferior mechanical properties and lacks aqueous stability. In this study, electrospun scaffolds from neat zein and zein blended with prepolymer and mildly cross-linked poly(glycerol sebacate) (PGS) were fabricated. Less toxic solvents like acetic acid and ethanol were used. The morphological, physiochemical and degradation properties of the as-spun fiber mats were determined. Neat zein and zein-PGS fiber mats with high zein concentration (24 wt % and 27 wt %) showed defect-free microstructures. The average fiber diameter decreased with increasing PGS amount from 0.7 ± 0.2 µm to 0.09 ± 0.03 µm. The addition of PGS to zein resulted in a seven-fold increase in ultimate tensile strength and a four-fold increase in failure strain, whereas the Young’s Modulus did not change significantly. Degradation tests in phosphate buffered saline revealed the morphological instability of zein containing fiber mats in contact with aqueous media. Therefore, the fibers were in situ cross-linked with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC)/N-Hydroxysuccinimide (NHS), which led to improved morphological stability in aqueous environment. The novel fibers have suitable properties for application in soft tissue engineering. MDPI 2018-03-08 /pmc/articles/PMC5869641/ /pubmed/29518041 http://dx.doi.org/10.3390/nano8030150 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vogt, Lena
Liverani, Liliana
Roether, Judith A.
Boccaccini, Aldo R.
Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering
title Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering
title_full Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering
title_fullStr Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering
title_full_unstemmed Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering
title_short Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering
title_sort electrospun zein fibers incorporating poly(glycerol sebacate) for soft tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869641/
https://www.ncbi.nlm.nih.gov/pubmed/29518041
http://dx.doi.org/10.3390/nano8030150
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