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Structure and Distribution of an Unrecognized Interstitium in Human Tissues
Confocal laser endomicroscopy (pCLE) provides real-time histologic imaging of human tissues at a depth of 60–70 μm during endoscopy. pCLE of the extrahepatic bile duct after fluorescein injection demonstrated a reticular pattern within fluorescein-filled sinuses that had no known anatomical correlat...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869738/ https://www.ncbi.nlm.nih.gov/pubmed/29588511 http://dx.doi.org/10.1038/s41598-018-23062-6 |
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author | Benias, Petros C. Wells, Rebecca G. Sackey-Aboagye, Bridget Klavan, Heather Reidy, Jason Buonocore, Darren Miranda, Markus Kornacki, Susan Wayne, Michael Carr-Locke, David L. Theise, Neil D. |
author_facet | Benias, Petros C. Wells, Rebecca G. Sackey-Aboagye, Bridget Klavan, Heather Reidy, Jason Buonocore, Darren Miranda, Markus Kornacki, Susan Wayne, Michael Carr-Locke, David L. Theise, Neil D. |
author_sort | Benias, Petros C. |
collection | PubMed |
description | Confocal laser endomicroscopy (pCLE) provides real-time histologic imaging of human tissues at a depth of 60–70 μm during endoscopy. pCLE of the extrahepatic bile duct after fluorescein injection demonstrated a reticular pattern within fluorescein-filled sinuses that had no known anatomical correlate. Freezing biopsy tissue before fixation preserved the anatomy of this structure, demonstrating that it is part of the submucosa and a previously unappreciated fluid-filled interstitial space, draining to lymph nodes and supported by a complex network of thick collagen bundles. These bundles are intermittently lined on one side by fibroblast-like cells that stain with endothelial markers and vimentin, although there is a highly unusual and extensive unlined interface between the matrix proteins of the bundles and the surrounding fluid. We observed similar structures in numerous tissues that are subject to intermittent or rhythmic compression, including the submucosae of the entire gastrointestinal tract and urinary bladder, the dermis, the peri-bronchial and peri-arterial soft tissues, and fascia. These anatomic structures may be important in cancer metastasis, edema, fibrosis, and mechanical functioning of many or all tissues and organs. In sum, we describe the anatomy and histology of a previously unrecognized, though widespread, macroscopic, fluid-filled space within and between tissues, a novel expansion and specification of the concept of the human interstitium. |
format | Online Article Text |
id | pubmed-5869738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58697382018-04-02 Structure and Distribution of an Unrecognized Interstitium in Human Tissues Benias, Petros C. Wells, Rebecca G. Sackey-Aboagye, Bridget Klavan, Heather Reidy, Jason Buonocore, Darren Miranda, Markus Kornacki, Susan Wayne, Michael Carr-Locke, David L. Theise, Neil D. Sci Rep Article Confocal laser endomicroscopy (pCLE) provides real-time histologic imaging of human tissues at a depth of 60–70 μm during endoscopy. pCLE of the extrahepatic bile duct after fluorescein injection demonstrated a reticular pattern within fluorescein-filled sinuses that had no known anatomical correlate. Freezing biopsy tissue before fixation preserved the anatomy of this structure, demonstrating that it is part of the submucosa and a previously unappreciated fluid-filled interstitial space, draining to lymph nodes and supported by a complex network of thick collagen bundles. These bundles are intermittently lined on one side by fibroblast-like cells that stain with endothelial markers and vimentin, although there is a highly unusual and extensive unlined interface between the matrix proteins of the bundles and the surrounding fluid. We observed similar structures in numerous tissues that are subject to intermittent or rhythmic compression, including the submucosae of the entire gastrointestinal tract and urinary bladder, the dermis, the peri-bronchial and peri-arterial soft tissues, and fascia. These anatomic structures may be important in cancer metastasis, edema, fibrosis, and mechanical functioning of many or all tissues and organs. In sum, we describe the anatomy and histology of a previously unrecognized, though widespread, macroscopic, fluid-filled space within and between tissues, a novel expansion and specification of the concept of the human interstitium. Nature Publishing Group UK 2018-03-27 /pmc/articles/PMC5869738/ /pubmed/29588511 http://dx.doi.org/10.1038/s41598-018-23062-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Benias, Petros C. Wells, Rebecca G. Sackey-Aboagye, Bridget Klavan, Heather Reidy, Jason Buonocore, Darren Miranda, Markus Kornacki, Susan Wayne, Michael Carr-Locke, David L. Theise, Neil D. Structure and Distribution of an Unrecognized Interstitium in Human Tissues |
title | Structure and Distribution of an Unrecognized Interstitium in Human Tissues |
title_full | Structure and Distribution of an Unrecognized Interstitium in Human Tissues |
title_fullStr | Structure and Distribution of an Unrecognized Interstitium in Human Tissues |
title_full_unstemmed | Structure and Distribution of an Unrecognized Interstitium in Human Tissues |
title_short | Structure and Distribution of an Unrecognized Interstitium in Human Tissues |
title_sort | structure and distribution of an unrecognized interstitium in human tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869738/ https://www.ncbi.nlm.nih.gov/pubmed/29588511 http://dx.doi.org/10.1038/s41598-018-23062-6 |
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