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Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples
OBJECTIVE: Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative PCR cycle threshold (Ct) values, th...
Autores principales: | Menu, Estelle, Mary, Charles, Toga, Isabelle, Raoult, Didier, Ranque, Stéphane, Bittar, Fadi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869780/ https://www.ncbi.nlm.nih.gov/pubmed/29587846 http://dx.doi.org/10.1186/s13104-018-3300-2 |
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