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Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples

OBJECTIVE: Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative PCR cycle threshold (Ct) values, th...

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Detalles Bibliográficos
Autores principales: Menu, Estelle, Mary, Charles, Toga, Isabelle, Raoult, Didier, Ranque, Stéphane, Bittar, Fadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869780/
https://www.ncbi.nlm.nih.gov/pubmed/29587846
http://dx.doi.org/10.1186/s13104-018-3300-2

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