Optimization of Brucella abortus Protocols for Downstream Molecular Applications

We compared the performances of various DNA extraction kits for their ability to recover Brucella abortus strain 19 inoculated into Brucella-free bovine tissues. Tissues were homogenized in a FastPrep bead homogenizer and extracted in triplicate by using one of five kits (Qiagen DNeasy, GE Illustra,...

Descripción completa

Detalles Bibliográficos
Autores principales: Hull, Noah, Miller, Jonathan, Berry, David, Laegreid, William, Smith, Ashley, Klinghagen, Callie, Schumaker, Brant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Clinical Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869842/
https://www.ncbi.nlm.nih.gov/pubmed/29436425
http://dx.doi.org/10.1128/JCM.01894-17

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869842/
https://www.ncbi.nlm.nih.gov/pubmed/29436425
http://dx.doi.org/10.1128/JCM.01894-17

Ejemplares similares