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Optimization of Brucella abortus Protocols for Downstream Molecular Applications
We compared the performances of various DNA extraction kits for their ability to recover Brucella abortus strain 19 inoculated into Brucella-free bovine tissues. Tissues were homogenized in a FastPrep bead homogenizer and extracted in triplicate by using one of five kits (Qiagen DNeasy, GE Illustra,...
Autores principales: | Hull, Noah, Miller, Jonathan, Berry, David, Laegreid, William, Smith, Ashley, Klinghagen, Callie, Schumaker, Brant |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869842/ https://www.ncbi.nlm.nih.gov/pubmed/29436425 http://dx.doi.org/10.1128/JCM.01894-17 |
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