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Species identification approach for both raw materials and end products of herbal supplements from Tinospora species

BACKGROUND: Nowadays herbal products used in traditional medicine are sold in processed forms and thus morphological authentication is almost impossible. With herbal industry rapidly growing size, consumer safety becomes an important issue that requires special attention. Identification of herbal sp...

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Autores principales: Osathanunkul, Maslin, Osathanunkul, Rossarin, Madesis, Panagiotis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870811/
https://www.ncbi.nlm.nih.gov/pubmed/29587839
http://dx.doi.org/10.1186/s12906-018-2174-0
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author Osathanunkul, Maslin
Osathanunkul, Rossarin
Madesis, Panagiotis
author_facet Osathanunkul, Maslin
Osathanunkul, Rossarin
Madesis, Panagiotis
author_sort Osathanunkul, Maslin
collection PubMed
description BACKGROUND: Nowadays herbal products used in traditional medicine are sold in processed forms and thus morphological authentication is almost impossible. With herbal industry rapidly growing size, consumer safety becomes an important issue that requires special attention. Identification of herbal species in the products is therefore needed. METHODS: Sequences from the selected regions (matK, rbcL, trnL and ITS1) were retrieved and analysed. Then the most suitable barcode was assessed for discrimination of T. crispa from closely related species by HRM analysis and used in authentication of commercial products. RESULTS: The ITS1 barcode was found to be the suitable primer as melting data from the HRM assay proved to be capable of distinguishing T. crispa from its related species. The developed protocol was then employed to authenticate medicinal products in powdered form. HRM analysis of all tested samples here revealed that five out of eight products contained not only the indicated species T. crispa but also other Tinospora, that have a high level of morphological similarity. CONCLUSION: Misrepresentation, poor packaging and inappropriate labeling of the tested medicinal herbal products are thought to be the reason of the results here. Using Bar-HRM with the ITS marker lead to success in authenticating the tested herbal products. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12906-018-2174-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-58708112018-03-29 Species identification approach for both raw materials and end products of herbal supplements from Tinospora species Osathanunkul, Maslin Osathanunkul, Rossarin Madesis, Panagiotis BMC Complement Altern Med Research Article BACKGROUND: Nowadays herbal products used in traditional medicine are sold in processed forms and thus morphological authentication is almost impossible. With herbal industry rapidly growing size, consumer safety becomes an important issue that requires special attention. Identification of herbal species in the products is therefore needed. METHODS: Sequences from the selected regions (matK, rbcL, trnL and ITS1) were retrieved and analysed. Then the most suitable barcode was assessed for discrimination of T. crispa from closely related species by HRM analysis and used in authentication of commercial products. RESULTS: The ITS1 barcode was found to be the suitable primer as melting data from the HRM assay proved to be capable of distinguishing T. crispa from its related species. The developed protocol was then employed to authenticate medicinal products in powdered form. HRM analysis of all tested samples here revealed that five out of eight products contained not only the indicated species T. crispa but also other Tinospora, that have a high level of morphological similarity. CONCLUSION: Misrepresentation, poor packaging and inappropriate labeling of the tested medicinal herbal products are thought to be the reason of the results here. Using Bar-HRM with the ITS marker lead to success in authenticating the tested herbal products. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12906-018-2174-0) contains supplementary material, which is available to authorized users. BioMed Central 2018-03-27 /pmc/articles/PMC5870811/ /pubmed/29587839 http://dx.doi.org/10.1186/s12906-018-2174-0 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Osathanunkul, Maslin
Osathanunkul, Rossarin
Madesis, Panagiotis
Species identification approach for both raw materials and end products of herbal supplements from Tinospora species
title Species identification approach for both raw materials and end products of herbal supplements from Tinospora species
title_full Species identification approach for both raw materials and end products of herbal supplements from Tinospora species
title_fullStr Species identification approach for both raw materials and end products of herbal supplements from Tinospora species
title_full_unstemmed Species identification approach for both raw materials and end products of herbal supplements from Tinospora species
title_short Species identification approach for both raw materials and end products of herbal supplements from Tinospora species
title_sort species identification approach for both raw materials and end products of herbal supplements from tinospora species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870811/
https://www.ncbi.nlm.nih.gov/pubmed/29587839
http://dx.doi.org/10.1186/s12906-018-2174-0
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