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Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer

MicroRNAs (miRNAs) are implicated in the development of nearly all cancers and may function as promising biomarkers for early detection, diagnosis and prognosis. We sought to investigate the role of serum miRNAs as potential diagnostic biomarkers or biomarkers of risk for early-stage bladder cancer....

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Autores principales: Lian, Jie, Lin, Shu-Hong, Ye, Yuanqing, Chang, David W., Huang, Maosheng, Dinney, Colin P., Wu, Xifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871085/
https://www.ncbi.nlm.nih.gov/pubmed/29599914
http://dx.doi.org/10.18632/oncotarget.24473
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author Lian, Jie
Lin, Shu-Hong
Ye, Yuanqing
Chang, David W.
Huang, Maosheng
Dinney, Colin P.
Wu, Xifeng
author_facet Lian, Jie
Lin, Shu-Hong
Ye, Yuanqing
Chang, David W.
Huang, Maosheng
Dinney, Colin P.
Wu, Xifeng
author_sort Lian, Jie
collection PubMed
description MicroRNAs (miRNAs) are implicated in the development of nearly all cancers and may function as promising biomarkers for early detection, diagnosis and prognosis. We sought to investigate the role of serum miRNAs as potential diagnostic biomarkers or biomarkers of risk for early-stage bladder cancer. First, we profiled global serum miRNAs in a pilot set of 10 non-muscle invasive bladder cancer (NMIBC) cases and 10 healthy controls matched on age, gender and smoking status. Eighty nine stably detectable miRNAs were selected for further testing and quantification by high-throughput Taqman analysis using the Fluidigm BioMark HD System to assess their association with NMIBC risk in both discovery and validation sets totaling 280 cases and 278 controls. We found miR-409-3p and six miRNAs expression ratios were significantly associated with risk of bladder cancer in both discovery and validation sets. Interestingly, we identified expression of miR-409-3p and miR-342-3p inversely correlated with age and age of onset of NMIBC. A risk score was generated based on the combination of three miRNA ratios (miR-29a-3p/miR-222-3p, miR-150-5p/miR-331-3p, miR-409-3p/miR-423-5p). In dichotomized analysis, we found individuals with high risk score showed increased risk of bladder cancer in the discovery, validation, and combined sets. Pathway enrichment analyses suggested altered miRNAs and cognate target genes are linked to the retinoid acid receptor (RAR) signaling pathway. Overall, these results suggested specific serum miRNA signatures may serve as noninvasive predictors of NMIBC risk. Biological insights underlying bladder cancer development based on the pathway enrichment analysis may reveal novel therapeutic targets for personalized medicine.
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spelling pubmed-58710852018-03-29 Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer Lian, Jie Lin, Shu-Hong Ye, Yuanqing Chang, David W. Huang, Maosheng Dinney, Colin P. Wu, Xifeng Oncotarget Research Paper MicroRNAs (miRNAs) are implicated in the development of nearly all cancers and may function as promising biomarkers for early detection, diagnosis and prognosis. We sought to investigate the role of serum miRNAs as potential diagnostic biomarkers or biomarkers of risk for early-stage bladder cancer. First, we profiled global serum miRNAs in a pilot set of 10 non-muscle invasive bladder cancer (NMIBC) cases and 10 healthy controls matched on age, gender and smoking status. Eighty nine stably detectable miRNAs were selected for further testing and quantification by high-throughput Taqman analysis using the Fluidigm BioMark HD System to assess their association with NMIBC risk in both discovery and validation sets totaling 280 cases and 278 controls. We found miR-409-3p and six miRNAs expression ratios were significantly associated with risk of bladder cancer in both discovery and validation sets. Interestingly, we identified expression of miR-409-3p and miR-342-3p inversely correlated with age and age of onset of NMIBC. A risk score was generated based on the combination of three miRNA ratios (miR-29a-3p/miR-222-3p, miR-150-5p/miR-331-3p, miR-409-3p/miR-423-5p). In dichotomized analysis, we found individuals with high risk score showed increased risk of bladder cancer in the discovery, validation, and combined sets. Pathway enrichment analyses suggested altered miRNAs and cognate target genes are linked to the retinoid acid receptor (RAR) signaling pathway. Overall, these results suggested specific serum miRNA signatures may serve as noninvasive predictors of NMIBC risk. Biological insights underlying bladder cancer development based on the pathway enrichment analysis may reveal novel therapeutic targets for personalized medicine. Impact Journals LLC 2018-02-12 /pmc/articles/PMC5871085/ /pubmed/29599914 http://dx.doi.org/10.18632/oncotarget.24473 Text en Copyright: © 2018 Lian et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Lian, Jie
Lin, Shu-Hong
Ye, Yuanqing
Chang, David W.
Huang, Maosheng
Dinney, Colin P.
Wu, Xifeng
Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer
title Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer
title_full Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer
title_fullStr Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer
title_full_unstemmed Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer
title_short Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer
title_sort serum micrornas as predictors of risk for non-muscle invasive bladder cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871085/
https://www.ncbi.nlm.nih.gov/pubmed/29599914
http://dx.doi.org/10.18632/oncotarget.24473
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