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A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice
Nonalcoholic fatty liver disease (NAFLD) is caused by ectopic fat accumulation in the liver. NAFLD is associated with hepatic inflammation and oxidative stress, resulting in nonalcoholic steatohepatitis (NASH) with advanced fibrosis. Placental extracts have been used to treat various chronic disease...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871096/ https://www.ncbi.nlm.nih.gov/pubmed/29599925 http://dx.doi.org/10.18632/oncotarget.24587 |
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author | Xu, Liang Nagata, Naoto Nagashimada, Mayumi Zhuge, Fen Ni, Yinhua Chen, Guanliang Kamei, Junzo Ishikawa, Hiroki Komatsu, Yasuhiko Kaneko, Shuichi Ota, Tsuguhito |
author_facet | Xu, Liang Nagata, Naoto Nagashimada, Mayumi Zhuge, Fen Ni, Yinhua Chen, Guanliang Kamei, Junzo Ishikawa, Hiroki Komatsu, Yasuhiko Kaneko, Shuichi Ota, Tsuguhito |
author_sort | Xu, Liang |
collection | PubMed |
description | Nonalcoholic fatty liver disease (NAFLD) is caused by ectopic fat accumulation in the liver. NAFLD is associated with hepatic inflammation and oxidative stress, resulting in nonalcoholic steatohepatitis (NASH) with advanced fibrosis. Placental extracts have been used to treat various chronic diseases due to their antioxidative effect. However, the effects of the extracts on the development of NASH have yet to be elucidated. Here, we demonstrated that supplementation with an oral porcine placental extract (PPE) attenuated lipid accumulation and peroxidation, insulin resistance, inflammatory and stress signaling, and fibrogenesis in the liver of NASH model mice fed a high-cholesterol and high-fat diet. The PPE reduced the number of M1-like liver macrophages, but increased the number of anti-inflammatory M2-like macrophages, resulting in a predominance of M2 over M1 macrophage populations in the liver of NASH mice. Accordingly, the PPE suppressed lipopolysaccharide-induced M1 polarization in isolated murine peritoneal macrophages, whereas it facilitated interleukin 4-induced M2 polarization. Furthermore, the PPE reduced the hepatic stellate cell (HSC) activation associated with the attenuated transforming growth factor-β/Smad3 signaling, both in the liver of NASH mice and in RI-T cells, a HSC line. The PPE may be a potential approach to prevent NASH by limiting lipid peroxidation, promoting M2 macrophage polarization, and attenuating HSC activation. |
format | Online Article Text |
id | pubmed-5871096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-58710962018-03-29 A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice Xu, Liang Nagata, Naoto Nagashimada, Mayumi Zhuge, Fen Ni, Yinhua Chen, Guanliang Kamei, Junzo Ishikawa, Hiroki Komatsu, Yasuhiko Kaneko, Shuichi Ota, Tsuguhito Oncotarget Research Paper Nonalcoholic fatty liver disease (NAFLD) is caused by ectopic fat accumulation in the liver. NAFLD is associated with hepatic inflammation and oxidative stress, resulting in nonalcoholic steatohepatitis (NASH) with advanced fibrosis. Placental extracts have been used to treat various chronic diseases due to their antioxidative effect. However, the effects of the extracts on the development of NASH have yet to be elucidated. Here, we demonstrated that supplementation with an oral porcine placental extract (PPE) attenuated lipid accumulation and peroxidation, insulin resistance, inflammatory and stress signaling, and fibrogenesis in the liver of NASH model mice fed a high-cholesterol and high-fat diet. The PPE reduced the number of M1-like liver macrophages, but increased the number of anti-inflammatory M2-like macrophages, resulting in a predominance of M2 over M1 macrophage populations in the liver of NASH mice. Accordingly, the PPE suppressed lipopolysaccharide-induced M1 polarization in isolated murine peritoneal macrophages, whereas it facilitated interleukin 4-induced M2 polarization. Furthermore, the PPE reduced the hepatic stellate cell (HSC) activation associated with the attenuated transforming growth factor-β/Smad3 signaling, both in the liver of NASH mice and in RI-T cells, a HSC line. The PPE may be a potential approach to prevent NASH by limiting lipid peroxidation, promoting M2 macrophage polarization, and attenuating HSC activation. Impact Journals LLC 2018-02-27 /pmc/articles/PMC5871096/ /pubmed/29599925 http://dx.doi.org/10.18632/oncotarget.24587 Text en Copyright: © 2018 Xu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xu, Liang Nagata, Naoto Nagashimada, Mayumi Zhuge, Fen Ni, Yinhua Chen, Guanliang Kamei, Junzo Ishikawa, Hiroki Komatsu, Yasuhiko Kaneko, Shuichi Ota, Tsuguhito A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice |
title | A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice |
title_full | A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice |
title_fullStr | A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice |
title_full_unstemmed | A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice |
title_short | A porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice |
title_sort | porcine placental extract prevents steatohepatitis by suppressing activation of macrophages and stellate cells in mice |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871096/ https://www.ncbi.nlm.nih.gov/pubmed/29599925 http://dx.doi.org/10.18632/oncotarget.24587 |
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