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The coronavirus nucleocapsid protein is ADP-ribosylated

ADP-ribosylation is a common post-translational modification, although how it modulates RNA virus infection is not well understood. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55 kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected c...

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Autores principales: Grunewald, Matthew E., Fehr, Anthony R., Athmer, Jeremiah, Perlman, Stanley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871557/
https://www.ncbi.nlm.nih.gov/pubmed/29199039
http://dx.doi.org/10.1016/j.virol.2017.11.020
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author Grunewald, Matthew E.
Fehr, Anthony R.
Athmer, Jeremiah
Perlman, Stanley
author_facet Grunewald, Matthew E.
Fehr, Anthony R.
Athmer, Jeremiah
Perlman, Stanley
author_sort Grunewald, Matthew E.
collection PubMed
description ADP-ribosylation is a common post-translational modification, although how it modulates RNA virus infection is not well understood. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55 kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected cells and in virions, which we identified as the viral nucleocapsid (N) protein. The N proteins of porcine epidemic diarrhea virus (PEDV), severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV were also ADP-ribosylated. ADP-ribosylation of N protein was also observed in cells exogenously expressing N protein by transduction using Venezuelan equine encephalitis virus replicon particles (VRPs). However, plasmid-derived N protein was not ADP-ribosylated following transient transfection but was ADP-ribosylated after MHV infection, indicating that this modification requires virus infection. In conclusion, we have identified a novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein.
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spelling pubmed-58715572019-04-01 The coronavirus nucleocapsid protein is ADP-ribosylated Grunewald, Matthew E. Fehr, Anthony R. Athmer, Jeremiah Perlman, Stanley Virology Article ADP-ribosylation is a common post-translational modification, although how it modulates RNA virus infection is not well understood. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55 kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected cells and in virions, which we identified as the viral nucleocapsid (N) protein. The N proteins of porcine epidemic diarrhea virus (PEDV), severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV were also ADP-ribosylated. ADP-ribosylation of N protein was also observed in cells exogenously expressing N protein by transduction using Venezuelan equine encephalitis virus replicon particles (VRPs). However, plasmid-derived N protein was not ADP-ribosylated following transient transfection but was ADP-ribosylated after MHV infection, indicating that this modification requires virus infection. In conclusion, we have identified a novel post-translation modification of the CoV N protein that may play a regulatory role for this important structural protein. Elsevier Inc. 2018-04 2017-12-01 /pmc/articles/PMC5871557/ /pubmed/29199039 http://dx.doi.org/10.1016/j.virol.2017.11.020 Text en © 2017 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Grunewald, Matthew E.
Fehr, Anthony R.
Athmer, Jeremiah
Perlman, Stanley
The coronavirus nucleocapsid protein is ADP-ribosylated
title The coronavirus nucleocapsid protein is ADP-ribosylated
title_full The coronavirus nucleocapsid protein is ADP-ribosylated
title_fullStr The coronavirus nucleocapsid protein is ADP-ribosylated
title_full_unstemmed The coronavirus nucleocapsid protein is ADP-ribosylated
title_short The coronavirus nucleocapsid protein is ADP-ribosylated
title_sort coronavirus nucleocapsid protein is adp-ribosylated
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871557/
https://www.ncbi.nlm.nih.gov/pubmed/29199039
http://dx.doi.org/10.1016/j.virol.2017.11.020
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