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Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment
Homeostasis of osteoclast formation from bone marrow macrophages (BMM) is regulated by paracrine signals of the neighbourhood bone cells particularly mesenchymal stem cells (MSC), osteoblasts and osteocytes (OC). Besides paracrine cues, collagen and glycosaminoglycan are involved in controlling bone...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871752/ https://www.ncbi.nlm.nih.gov/pubmed/29593232 http://dx.doi.org/10.1038/s41598-018-23532-x |
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author | Elango, Jeevithan Sanchez, Christelle de Val, José Eduardo Maté Sánchez Henrotin, Yves Wang, Shujun Motaung, Keolebogile Shirley Caroline Mamotswere Guo, Ruihua Wang, Chunxiao Robinson, Jeyashakila Regenstein, Joe M. Bao, Bin Wu, Wenhui |
author_facet | Elango, Jeevithan Sanchez, Christelle de Val, José Eduardo Maté Sánchez Henrotin, Yves Wang, Shujun Motaung, Keolebogile Shirley Caroline Mamotswere Guo, Ruihua Wang, Chunxiao Robinson, Jeyashakila Regenstein, Joe M. Bao, Bin Wu, Wenhui |
author_sort | Elango, Jeevithan |
collection | PubMed |
description | Homeostasis of osteoclast formation from bone marrow macrophages (BMM) is regulated by paracrine signals of the neighbourhood bone cells particularly mesenchymal stem cells (MSC), osteoblasts and osteocytes (OC). Besides paracrine cues, collagen and glycosaminoglycan are involved in controlling bone homeostasis. Towards this approach, different molecular weight collagens were reacted with MSC, OC and BMM to understand the bone homeostasis activity of collagen. The up-regulating effect of collagens on osteogenic cell growth was confirmed by the presence of mineralized nodules in the osteoblastogenic lineage cells and increased osteogenic stimulatory gene expression. The decreased BMM-derived TRAP+ osteoclasts number and osteoclastogenic regulatory gene expression of OC could demonstrate the exploitive osteoclastogenic activity of collagens. Osteoclastogenesis from BMM was triggered by paracrine cues of OC in some extend, but it was down-regulated by collagen. Overall, the effect of collagen on osteoclastogenesis and osteoblastogenesis may depend on the molecular weight of collagens, and collagen suppresses osteoclastogenesis, at least in part by downregulating the secretion of cytokines in OC. |
format | Online Article Text |
id | pubmed-5871752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58717522018-04-02 Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment Elango, Jeevithan Sanchez, Christelle de Val, José Eduardo Maté Sánchez Henrotin, Yves Wang, Shujun Motaung, Keolebogile Shirley Caroline Mamotswere Guo, Ruihua Wang, Chunxiao Robinson, Jeyashakila Regenstein, Joe M. Bao, Bin Wu, Wenhui Sci Rep Article Homeostasis of osteoclast formation from bone marrow macrophages (BMM) is regulated by paracrine signals of the neighbourhood bone cells particularly mesenchymal stem cells (MSC), osteoblasts and osteocytes (OC). Besides paracrine cues, collagen and glycosaminoglycan are involved in controlling bone homeostasis. Towards this approach, different molecular weight collagens were reacted with MSC, OC and BMM to understand the bone homeostasis activity of collagen. The up-regulating effect of collagens on osteogenic cell growth was confirmed by the presence of mineralized nodules in the osteoblastogenic lineage cells and increased osteogenic stimulatory gene expression. The decreased BMM-derived TRAP+ osteoclasts number and osteoclastogenic regulatory gene expression of OC could demonstrate the exploitive osteoclastogenic activity of collagens. Osteoclastogenesis from BMM was triggered by paracrine cues of OC in some extend, but it was down-regulated by collagen. Overall, the effect of collagen on osteoclastogenesis and osteoblastogenesis may depend on the molecular weight of collagens, and collagen suppresses osteoclastogenesis, at least in part by downregulating the secretion of cytokines in OC. Nature Publishing Group UK 2018-03-28 /pmc/articles/PMC5871752/ /pubmed/29593232 http://dx.doi.org/10.1038/s41598-018-23532-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Elango, Jeevithan Sanchez, Christelle de Val, José Eduardo Maté Sánchez Henrotin, Yves Wang, Shujun Motaung, Keolebogile Shirley Caroline Mamotswere Guo, Ruihua Wang, Chunxiao Robinson, Jeyashakila Regenstein, Joe M. Bao, Bin Wu, Wenhui Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment |
title | Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment |
title_full | Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment |
title_fullStr | Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment |
title_full_unstemmed | Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment |
title_short | Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment |
title_sort | cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871752/ https://www.ncbi.nlm.nih.gov/pubmed/29593232 http://dx.doi.org/10.1038/s41598-018-23532-x |
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