A DNA-Based Assay for Digoxin Detection

The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of th...

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Autores principales: Kjelstrup, Michael V., Nielsen, Line D. F., Hansen-Bruhn, Malthe, Gothelf, Kurt V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872067/
https://www.ncbi.nlm.nih.gov/pubmed/29509662
http://dx.doi.org/10.3390/bios8010019
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author Kjelstrup, Michael V.
Nielsen, Line D. F.
Hansen-Bruhn, Malthe
Gothelf, Kurt V.
author_facet Kjelstrup, Michael V.
Nielsen, Line D. F.
Hansen-Bruhn, Malthe
Gothelf, Kurt V.
author_sort Kjelstrup, Michael V.
collection PubMed
description The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring.
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spelling pubmed-58720672018-03-29 A DNA-Based Assay for Digoxin Detection Kjelstrup, Michael V. Nielsen, Line D. F. Hansen-Bruhn, Malthe Gothelf, Kurt V. Biosensors (Basel) Article The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring. MDPI 2018-03-06 /pmc/articles/PMC5872067/ /pubmed/29509662 http://dx.doi.org/10.3390/bios8010019 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kjelstrup, Michael V.
Nielsen, Line D. F.
Hansen-Bruhn, Malthe
Gothelf, Kurt V.
A DNA-Based Assay for Digoxin Detection
title A DNA-Based Assay for Digoxin Detection
title_full A DNA-Based Assay for Digoxin Detection
title_fullStr A DNA-Based Assay for Digoxin Detection
title_full_unstemmed A DNA-Based Assay for Digoxin Detection
title_short A DNA-Based Assay for Digoxin Detection
title_sort dna-based assay for digoxin detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872067/
https://www.ncbi.nlm.nih.gov/pubmed/29509662
http://dx.doi.org/10.3390/bios8010019
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