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Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection
D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophreni...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872068/ https://www.ncbi.nlm.nih.gov/pubmed/29509674 http://dx.doi.org/10.3390/bios8010020 |
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author | Campos-Beltrán, Diana Konradsson-Geuken, Åsa Quintero, Jorge E. Marshall, Lisa |
author_facet | Campos-Beltrán, Diana Konradsson-Geuken, Åsa Quintero, Jorge E. Marshall, Lisa |
author_sort | Campos-Beltrán, Diana |
collection | PubMed |
description | D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/µM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 µM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies. |
format | Online Article Text |
id | pubmed-5872068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58720682018-03-29 Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection Campos-Beltrán, Diana Konradsson-Geuken, Åsa Quintero, Jorge E. Marshall, Lisa Biosensors (Basel) Article D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/µM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 µM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies. MDPI 2018-03-06 /pmc/articles/PMC5872068/ /pubmed/29509674 http://dx.doi.org/10.3390/bios8010020 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Campos-Beltrán, Diana Konradsson-Geuken, Åsa Quintero, Jorge E. Marshall, Lisa Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection |
title | Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection |
title_full | Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection |
title_fullStr | Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection |
title_full_unstemmed | Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection |
title_short | Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection |
title_sort | amperometric self-referencing ceramic based microelectrode arrays for d-serine detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872068/ https://www.ncbi.nlm.nih.gov/pubmed/29509674 http://dx.doi.org/10.3390/bios8010020 |
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