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Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity
Ammonia (NH(3))-oxidizing bacteria (AOB) derive total energy for life from the multi-electron oxidation of NH(3) to nitrite (NO(2)(–)). One obligate intermediate of this metabolism is hydroxylamine (NH(2)OH), which can be oxidized to the potent greenhouse agent nitrous oxide (N(2)O) by the AOB enzym...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Royal Society of Chemistry
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872139/ https://www.ncbi.nlm.nih.gov/pubmed/29629106 http://dx.doi.org/10.1039/c7sc03450d |
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author | Vilbert, Avery C. Caranto, Jonathan D. Lancaster, Kyle M. |
author_facet | Vilbert, Avery C. Caranto, Jonathan D. Lancaster, Kyle M. |
author_sort | Vilbert, Avery C. |
collection | PubMed |
description | Ammonia (NH(3))-oxidizing bacteria (AOB) derive total energy for life from the multi-electron oxidation of NH(3) to nitrite (NO(2)(–)). One obligate intermediate of this metabolism is hydroxylamine (NH(2)OH), which can be oxidized to the potent greenhouse agent nitrous oxide (N(2)O) by the AOB enzyme cytochrome (cyt) P460. We have now spectroscopically characterized a 6-coordinate (6c) {FeNO}(7) intermediate on the NH(2)OH oxidation pathway of cyt P460. This species has two fates: it can either be oxidized to the {FeNO}(6) that then undergoes attack by NH(2)OH to ultimately generate N(2)O, or it can lose its axial His ligand, thus generating a stable, off-pathway 5-coordinate (5c) {FeNO}(7) species. We show that the wild type (WT) cyt P460 exhibits a slow nitric oxide (NO)-independent conversion (k(His-off) = 2.90 × 10(–3) s(–1)), whereas a cross-link-deficient Lys70Tyr cyt P460 mutant protein underwent His dissociation via both a NO-independent (k(His-off) = 3.8 × 10(–4) s(–1)) and a NO-dependent pathway [k(His-off(NO)) = 790 M(–1) s(–1)]. Eyring analyses of the NO-independent pathways for these two proteins revealed a significantly larger (ca. 27 cal mol(–1) K(–1)) activation entropy (ΔS(‡)) in the cross-link-deficient mutant. Our results suggest that the Lys–heme cross-link confers rigidity to the positioning of the heme P460 cofactor to avoid the fast NO-dependent His dissociation pathway and subsequent formation of the off-pathway 5c {FeNO}(7) species. The relevance of these findings to NO signaling proteins such as heme-nitric oxide/oxygen binding (H-NOX) is also discussed. |
format | Online Article Text |
id | pubmed-5872139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-58721392018-04-06 Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity Vilbert, Avery C. Caranto, Jonathan D. Lancaster, Kyle M. Chem Sci Chemistry Ammonia (NH(3))-oxidizing bacteria (AOB) derive total energy for life from the multi-electron oxidation of NH(3) to nitrite (NO(2)(–)). One obligate intermediate of this metabolism is hydroxylamine (NH(2)OH), which can be oxidized to the potent greenhouse agent nitrous oxide (N(2)O) by the AOB enzyme cytochrome (cyt) P460. We have now spectroscopically characterized a 6-coordinate (6c) {FeNO}(7) intermediate on the NH(2)OH oxidation pathway of cyt P460. This species has two fates: it can either be oxidized to the {FeNO}(6) that then undergoes attack by NH(2)OH to ultimately generate N(2)O, or it can lose its axial His ligand, thus generating a stable, off-pathway 5-coordinate (5c) {FeNO}(7) species. We show that the wild type (WT) cyt P460 exhibits a slow nitric oxide (NO)-independent conversion (k(His-off) = 2.90 × 10(–3) s(–1)), whereas a cross-link-deficient Lys70Tyr cyt P460 mutant protein underwent His dissociation via both a NO-independent (k(His-off) = 3.8 × 10(–4) s(–1)) and a NO-dependent pathway [k(His-off(NO)) = 790 M(–1) s(–1)]. Eyring analyses of the NO-independent pathways for these two proteins revealed a significantly larger (ca. 27 cal mol(–1) K(–1)) activation entropy (ΔS(‡)) in the cross-link-deficient mutant. Our results suggest that the Lys–heme cross-link confers rigidity to the positioning of the heme P460 cofactor to avoid the fast NO-dependent His dissociation pathway and subsequent formation of the off-pathway 5c {FeNO}(7) species. The relevance of these findings to NO signaling proteins such as heme-nitric oxide/oxygen binding (H-NOX) is also discussed. Royal Society of Chemistry 2017-11-07 /pmc/articles/PMC5872139/ /pubmed/29629106 http://dx.doi.org/10.1039/c7sc03450d Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
spellingShingle | Chemistry Vilbert, Avery C. Caranto, Jonathan D. Lancaster, Kyle M. Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity |
title | Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity
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title_full | Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity
|
title_fullStr | Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity
|
title_full_unstemmed | Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity
|
title_short | Influences of the heme-lysine crosslink in cytochrome P460 over redox catalysis and nitric oxide sensitivity
|
title_sort | influences of the heme-lysine crosslink in cytochrome p460 over redox catalysis and nitric oxide sensitivity |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872139/ https://www.ncbi.nlm.nih.gov/pubmed/29629106 http://dx.doi.org/10.1039/c7sc03450d |
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